Rapid identification of candida species by dna fingerprinting with pcr,candidiasis symptoms in cats,do panty liners cause yeast infection - Videos Download
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Microsatellite DNA Identification and genotyping of Candida albicans from Lebanese clinical Isolates. Use of molecular methods in identification of Candida species and evaluation of fluconazole resistance. Identification of medically important yeasts using PCR-based detection of DNA sequence polymorphisms in the internal transcribed spacer 2 region of rRNA genes.
Molecular identification of Candida albicans isolated from the oncology patients at four university hospitals in Mazandaran province (2005-6). Comparison of different methods for extraction of mitochondrial DNA from human pathogenic yeasts. Evaluation of internal transcribed spacer region of ribosomal DNA sequence analysis for molecular characterization of Candida albicans and Candida dubliniensis isolates from HIV-infected patients. Identifidcation of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA.
Genetic identification of Candida species in HIV-positive patients using the polymerase chain restriction fragment length polymorphism analysis of its DNA.
Molecular and phenotypic characterization of genotypic Candida albicans subgroups and comparison with Candida dubliniensis and Candida stellatoidea.
Traditional methods that are used to identify clinical isolates of Candida species are time-consuming and not appropriate for rapid, accurate and reliable identification.
DNA pyrosequencing-based identification of pathogenic Candida species by using the internal transcribed spacer 2 region. This is thought to be the result of the increase in size of populations at risk such as, cancer patients, HIV-infected patients, transplant recipients and those receiving immunosuppressive and broad-spectrum antibiotic therapy.Although Candida albicans remains the most frequent cause of Candidiasis, the incidence of the disease caused by other species of Candida has been increasing steadily. Definitive identifications, leading to significant delays in appropriate patients′ management due to the significant morbidity and mortality are associated with invasive Candida infections. The clinical strains were isolated from, lip, throat and tongue of patients with cancer in four university hospitals, Mazandaran Province [Table - 1]. The size of DNA fragments determined directly with comparison of molecular size marker and distinct banding patterns which demonstrated in similar studies.ResultsIn this study, we apply a PCR-RFLP for identification of the medically important Candida spp. Candida identification kits based on assimilation require at least 1-5 days for identification at the species level, yet inaccuracies are always possible. This procedure with high discrimination power is used in species diagnosis especially in the epidemiological studies to evaluate route of transmission, as well as to choose appropriate antifungal drugs.
These methods can directly detect the presence of fungi with high degree of specificity and sensitivity. These regions are appropriate for diagnosis, identification, taxonomy and phylogeny of fungi, which are medically important.
PCR-RFLP method has advantages over other molecular techniques, such as restriction fragment length polymorphism with genomic DNA and electrophoretic karyotyping, in being simple and quick. Pinto et al,  used universal primers ITS1 and ITS4 for the amplification of ITS1 and ITS2 regions, including 5.8S subunit genes and digested it with eight restriction enzymes.
Mirhendi et al,  using PCR-RFLP method and fungus-specific universal primers (ITS1 and ITS2) were able to amplify the ITS regions of all yeasts tested, and then PCR amplicons were digested with MspI.
Similar to other papers, , the same results were obtained by CHROMagar and PCR-RFLP methods in detecting different Candida spp, however in our study a couple of isolate showed different results using CHROMagar due to undetermined color.
This finding is a conclusive reason to apply molecular methods for determination and identification of medically important Candida spp. This method is useful for clinical and epidemiological investigation both mucocutaneous and systemic formsSince the methods used in this study are easy to perform and just require standard molecular biology equipment, there is no reason to limit the use of PCR in research laboratory. Another advantage of this method, comparing the other molecular methods, is the speed of species identification.
In conclusion, this genotyping method has been appraised to identify Candida spp.Williams et al, showed that the results were stable after both repeated sub-culturing and storing the isolate for at least six months.
This profile was also recommended as an easy and quick method for discrimination between two morphologically similar species, C.
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