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CasePerformance: Tribulus Part II - The Human Studies: Testosterone Booster or Marketing Hype?
Tribulus has been hailed as Mother Nature’s (or should I say Father Earth’s?) very own testosterone boosting, muscle popping, libido driving super star supplement.
Although some research indicates that it does lead to small, acute spikes in androgen levels (ie – testosterone & DHT), just as many show it to have little to no effect on enhancing production of these hormones. However, despite some interesting findings in animals, we all know that animal studies do not always play nice and perfectly translate over to humans. For those only interested in the potential of Tribulus supplementation to booster your sex life, scroll down to the bottom of the article. Above are the androgenic pathways; As you can see, the end products of these pathways include testosterone, estrogen and DHT.
Dihydrotestosterone (DHT) - Bigger, Stronger, Faster or just Balder, Fatter and Unhealthier?
Before we get into the research, it’s important to ask a question that most manufacturers hope you never do… Namely, does this herbal supplement actually contain the active ingredients associated with its purported effects??? With that being said, I guess it should not come as a surprise to you that not all Tribulus terrestris contains equal amounts of PTN.
Okay, now that we’ve discussed the androgenic pathways, how Tribulus may fit into the picture and the affects of tribulus supplementation in animals, we’re back to square one. At the conclusion of the study Antonio et al found no significant differences between and within the groups with respect to improved mood states, overall body weight and % body fat.21 For reference, % body fat was assessed via the gold standard technique, underwater weighing. For reference, food intake did not appear to confound results of this study completed by Rogerson et al22. Did you notice the outrageous strength gains over the course of 5 weeks in the study by Rogerson et al?22 If so, you’re not alone as one of the first things I noticed was that in both groups, the bench press shot up 26-33 lbs (12-15 kg), seated row by 35-39 lbs (16-18 kg), deadlift 82-95 lbs (37-43 kg) and leg press by 231-264 lbs (105-120 kg). The most widely referenced study to promote both the testosterone boosting, athletic enhancing abilities of tribulus is that completed by Milasius et al.25 In their study, 32 athletes, between the ages of 20-22 were separated into 2 separate groups. At the conclusion of the 20 day period it was found that tribulus significantly increased anaerobic alactic muscular power (6%), alactate glycolyte working capacity (6%), and VO2 at aneorobic threshold (11%). Second, normal dietary practices by the study’s participants were not discussed in the study.
Fourth, the study’s authors fail to provide reasoning for the unbalanced distribution of participants in each group (control – 12; experimental – 20). Fifth, many of the variables were just assessed in the experimental group (VO2, testosterone, etc).
Finally, this is the first and only study that I’ve come across that reported changes in testosterone levels based off its Km value vs. So far we’ve discussed the research in regards to Tribulus and changes in measurements of athletic performance. Thus, I’d like to shift our focus towards research presented by Neychev & Mitev, who I believe are the only individuals who have examined and published their findings in a peer reviewed journal on testosterone production and Tribulus supplementation. With respect to the effects of Tribulus on sexual activity and function in humans, I was unable to find any research in peer reviewed journals.
22 Rogerson S, Riches CJ, Jennings C, Weatherby RP, Meir RA, Marshall-Gradisnik SM.The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players.
32 Bhasin S, Woodhouse L, Casaburi R, Singh AB, Bhasin D, Berman N, Chen X, Yarasheski KE, Magliano L, Dzekov C, Dzekov J, Bross R, Phillips J, Sinha-Hikim I, Shen R, Storer TW.
33 Gray PB, Singh AB, Woodhouse LJ, Storer TW, Casaburi R, Dzekov J, Dzekov C, Sinha-Hikim I, Bhasin S.
34 Bhasin S, Woodhouse L, Casaburi R, Singh AB, Mac RP, Lee M, Yarasheski KE, Sinha-Hikim I, Dzekov C, Dzekov J, Magliano L, Storer TW.
This information is not intended to take the place of medical advice.Please check with your health care providers prior to starting any new dietary or exercise program. About the Author: Sean Casey is a graduate of the University of Wisconsin-Madison with degrees in both Nutritional Science-Dietetics and Kinesiology-Exercise Physiology. Delivery times may vary, especially during peak periods and will depend on when your payment clears - opens in a new window or tab. By clicking Confirm bid you commit to buy this item from the seller if you are the winning bidder.


Import charges previously quoted are subject to change if you increase you maximum bid amount. However, an obvious, but often forgotten question, one must remember to ask themselves prior to purchase – “Is there any evidence it actually works?” In Part II of our examination of Tribulus, we examine its effects in humans. Cool looking plant but will it actually increase your testosterone levels, physical performance or sex drive?
Then, we turned to our furry, fuzzy animal friends and examined how they responded to the supplement. With respect to the purported aphrodisiac properties of Tribulus, slight to moderate improvements in sex drive have been noted in rats post supplementation. In a study completed by Dinchev et al, researchers examined the PTN content of Tribulus terresties obtained from various parts of the world (Bulgaria, Greece, Serbia, Macedonia, Turkey, Georgia, Iran, Vietnam and India).27 PTN was highest in that of European and western Asia. The mean ages of the participants were between 20-22 years and they had averaged 3 resistance training sessions per week during the previous year. With respect to changes in exercise performance, both groups experienced significant relative gains in lower body endurance as determined by “repping” out on a leg press machine set at 200% body weight (Figure 3). Although both groups increased fat free mass (ie-lean body mass) over the course of the study, no significant differences existed between those receiving the placebo vs. These strength gains would be impressive even for “newbies” who naturally have great gains when first starting a training program. Ideally the participants would have evenly been distributed such that 16 were in each group. Of course there will be no change in these variables amongst the control group if you don’t measure them!!! However, although 2 of the above studies examined the effects of Tribulus on testosterone, neither of them directly assessed total testosterone production (Which has it’s own caveats – Refer to SHBG section of Part I). Well, yes, testosterone is essential for sex drive and those suffering from hypogonadism (ie-low testosterone levels) do see improvements in this area upon starting up with testosterone replacement.2930. Testosterone Levels after 16 weeks of GnRH blocker and various testosterone enthanate doses in younger men. Thus, the serum testosterone levels shown in Figure 6 were, theoretically speaking, at their lowest points during the week. Testosterone Levels after 16 weeks of GnRH blocker and various testosterone enthanate doses in older men.
Even if it did, the likelihood of these spikes actually resulting in increased sexual activity or function is likely not going to happen. The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males. Determining the magnitude of treatment effects in strength training research through the use of the effect size. Short term impact of Tribulus terrestris intake on doping control analysis of endogenous steroids. The influence of the Tribulus terrestris extract on the parameters of the functional preparedness and athletes’ organism homeostasis. Distribution of steroidal saponins in Tribulus terrestris from different geographical regions. Determination of steroidal saponins in Tribulus terrestris by reversed-phase high-performance liquid chromatography and evaporative light scattering detection. Androgens play a pivotal role in maintaining penile tissue architecture and erection: a review. Dose-dependent effects of testosterone on sexual function, mood, and visuospatial cognition in older men. Older men are as responsive as young men to the anabolic effects of graded doses of testosterone on the skeletal muscle. Proerectile pharmacological effects of Tribulus terrestris extract on the rabbit corpus cavernosum.
The hormonal effects of Tribulus terrestris and its role in the managementof male erectile dysfunction – an evaluation using primates, rabbit and rat.
CasePerformance is not responsible for the outcome of any decision made based off the information presented in this article. If you reside in an EU member state besides UK, import VAT on this purchase is not recoverable. Testosterone is the most plentiful androgen in men, and women also produce androgens in the ovaries and adrenal cortex. Skimping on sleep may lower testosterone levels, so make sure you get 7 or 8 hours a night.
Testosterone is a naturally occurring steroid hormone responsible for proper growth of sex organs.
One highly questionable study (from a scientific methodology standpoint), indicated that Tribulus supplementation did positively influence various aspects of athletic performance. According to some supplement companies, this is to be expected as Tribulus is Mother Nature’s, (or should I say Father Earth’s?), very own testosterone boosting, muscle popping, libido driving super star supplement … Or is it?
With respect to its anabolic effects, one study has shown significant weight gain post supplementation. Thus, in this, the concluding part of our Tribulus “mini” series, I’d like to examine the research in regards to its effect on androgenic hormones production, exercise performance and sex drive in humans.
PLEASE NOTE this is a simplified version of the interaction between the various androgenic hormones.


Additional pertinent info – Although the origin of the supplement was not listed, the supplements were standardized to 45% saponins and protodyosin (PTN).
For those not familiar with Km values, this has to do with the speed at which “enzymes” carry out a given reaction. In addition, building off what I mentioned in my first point, if participants were left to do “whatever” on their own, I’m guessing that those in the tribulus group would naturally work harder simply because they would want to take advantage of any benefits tribulus may offer them. The fewer people in a group, the more “extreme” results have to be in order to prove significance. Thus, it’s hard to put into context the importance of this testosterone increase when comparing it to other pieces of scientific literature. Despite the great range in circulating total testosterone levels, none of the groups experienced any significant changes in sexual activity or sexual desire scores. Mitev The aphrodisiac herb Tribulus terrestris does not influence the androgen production in young men. He has also spent time as an intern physical preparation coach at the International Performance Institute in Bradenton, FL.
Latest news, updates, price, drivers, reviews, and more for Testosterone Blockers for Men MTF. The conversion of testosterone to DHT, or dihydrotestosterone, is one of the main causes of hair loss. Risk is greater in transgender men who are obese, smoke, or have COPD (Chronic Obstructive Pulmonary Disease). Awesome action games, puzzle games, adventure games, multiplayer games, skill games & best action games.
However, in other more rigorously designed studies, Tribulus had no effect on testosterone production, muscular strength, strength endurance, or body composition changes (ie – % body fat, etc). Ganzera et al also found that Tribulus, obtained from southeast Asia (China), has lower PTN content than that found in Bulgaria.28 The PTN content of your supplement can also vary extensively depending on which part of the plant is included in the supplement.
All individuals had a minimum of 12 months resistance training experience and although considerable variation was present, the mean training age for each group was similar (33-36 months).
According to Rogerson et al, they all had a minimum of 12 months of lifting experience, particularly in the lifts that were used in the testing of this study. In general, when more substrate is available (in this case testosterone), Km values are higher.
Likewise, the more people in a group, the less “extreme” results have to be in order to show statistical significance.
In addition, the authors did not elaborate on why the used the Km or how they obtained the Km value. On the flip side of the equation, there is an abundance of evidence, in both human and animal studies, that androgenic hormone levels are not affected post Tribulus supplementation.
This held true in both those who saw their circulating total testosterone (and free testosterone – data not shown) drop below or rise above the “normal” circulating levels. Why do these differences exist between older and younger men with respect to testosterone and sexual activity? Multiple studies have indicated that the highest concentration of PTN is found in the leaves of the Tribulus plant, followed by the fruits and finally the stem.2728. In regards to their diet, kcal and macronutrient distribution of the participants’ diet did not differ between or within groups throughout the study. Prior to the start of the preseason, they did have a 6 week off season break in which they “stayed active” but did not have any “formal structured training” during the downtime. The control group experienced no significant differences with respect to anaerobic alactic muscular power or alactate glycolyte working capacity. Thus, based off how this study is set up, it’s more likely to see statistically significant changes pre vs.
Generally speaking, research articles will discuss the methods (going back to point 1) in which they obtained various lab values. Taking all this into account, Tribulus once again falls short on the claims made by supplement manufacturers. While at these locations he had the opportunity to train football, soccer, baseball, golf and tennis athletes. As discussed in Part I of our look at Tribulus, there is weak to moderate evidence that the supplement did enhance sexual behaviors in rats.
Although the research showing improved athletic performance in athletes post tribulus supplementation is interesting, there are a lot of MAJOR caveats in regards to the research completed by Milasius et al. There have not been any studies in human or animals that have directly assessed changes in blood flow following tribulus supplementation. Sean is also active in the field of sports nutrition where he has consulted with a wide variety of organizations including both elite (NFL’s Jacksonville Jaguars) and amateur athletic teams.
That said, based off various lines of evidence discussed within this article, it does not appear that Tribulus lives up to it’s aphrodisiac hype. In most studies that have uneven groups, the researchers will provide reasoning behind it (ie- people had to drop out because of reasons X, Y, Z).
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