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What will you make with Biology?
Banana Perfume
Red Bio-Paint
Red Bio-Paint
LB Agar Powder
Antibiotics
Materials
 5. Recover Cells
Nitrile Gloves
 3. Make Cells Competent
 1. Make LB Agar Plates
 6. Plate Cells
Petri Dishes
Sterile Water
Start Making LB Agar Plates
Engineer-It kit 
 4. Transform Cells
 2. Grow Blank Cells
 7. Incubate Cells
Its important to use sterile water when you bioengineer, because if there are any other microorganisms in the water, they’ll grow too!
Got it
Agar Powder
LB is food for bacteria. Agar makes the LB jelly-like so you grow bacteria on the surface of it. LB contains sugar (glucose), tryptone (amino acids), and yeast extracts.
Petri dishes are the containers that you grow bacteria in. As you’ll see, you pour different kinds of molten LB agar into the dishes.
Antibiotics are added to molten LB agar to make the media “selective”. You need selective plates when you grow engineered bacteria.
When bioengineering you should wear gloves (latex, nitrile). You do this to protect you from your experiments and your experiments from you!
Microwave
You need a microwave to boil your sterile water. Having boiled sterile water will make sure your LB Agar powder will dissolve
Go
Drag the sterile water into the microwave and press GO!
Your water is boiling!
Next Step
Remove Water From Microwave
Put on your Purple Nitrile Gloves
Sorry friend, this is as far as you can go using a MOBILE Device. In step 4 I need to give you a cool thing I made, so hurry and get a PC/Laptop!
What is made with Genetic Engineering?
Pour the LB powder into the boiling water by dragging over your boiling water
Restart
Pour
Reminder
Next
2.  Grow Blank Cells
Medical Diabetes is a medical condition that is often treated with insulin, but where does that insulin come from? Until the 1970s, insulin was collected from the pancreas of dead pigs and horses. The organs were dried then ground up, and the insulin (naturally produced in the pancreas was isolated and injected into patients. This process helped many people control their diabetes but also led to allergic reactions and impure samples of insulin.  But in the 1970s scientists identified and isolated the DNA snippet that encoded human insulin! They put this snippet into a plasmid, transformed that plasmid into a bacterial cell and then cultured it – just as you did in this simulation. The engineered microbe was producing a safe human insulin: Humulin. In 2006, an estimated 150,000,00 vials of insulin were produced for millions of patients (ref). Many of today’s medicines are produced with these bioengineering techniques. Bacteria and yeast cells are grown in large bioreactors to produce improved insulin, several cancer therapies, and other medicines. 
Today, most colours and dyes are made using industrial chemistry. This means lots of heat, energy, and in many cases, a large impact on the environment and the health of people creating them.
Bioengineering is set to help individuals and industries make inks, dyes, and pigments sustainably, from sources that were never before possible. What if we could make a safer, better RED?! Its your job to genetically engineer E. coli bacteria to produce a red pigment that comes from coral. In this exercise you will get a tube of DNA from your friend. This DNA is a program she developed so that when you insert it into bacteria, it will be produced by the bacteria as it grows- a sustainable pigment!
Bioengineer Red pigment
We need more RED !
Plants can be bioengineered too. Several food crops have been engineered to produce more nutrient rich, drought resistant, or pest resistant varieties. To engineer plants, a DNA plasmid is modified to express a specific function, such as vitamin production. The DNA is transformed into a bacterial cell, just as you did in this simulation. The plant is then mixed with this transformed bacteria and under special conditions, the DNA from the bacterial cell will enter the plant cells. The plant can then make the vitamin or whatever the DNA program directs.  The first transgenic plants were developed in the 1980s. These were antibiotic resistant tobacco plants. Today millions of people rely on genetically modified foods every day, but the technology remains controversial and people are concerned with the safety of foods in terms of human health and our planet’s ecosystem.
Until recently, the only way to make plastics was to produce them from fossil fuels. But these days, biology can be used to make materials like some bioplastics and biorubbers. Bacteria and yeast cells have been engineered with DNA that can build small carbon molecules inside the cells. These carbon molecules are the building blocks that can be connected together to make bioplastics and biorubbers. 
Medical When you think of energy, you probably think of coal or nuclear power plants. However, in recent years living organisms have been used to generate energy, usually in the form of liquid fuels.  Bacteria, yeast, and algae are engineered with new DNA to produce energy rich compounds: Attempts to produce a gasoline like molecule, butanol, in bacteria are underway. Ethanol can be produced in yeast cells for use in vehicles, and algae are engineered to produce kerosene, a component of jet fuel.. Researchers are also engineering bacteria to directly produce electricity - Look up microbial fuel cells to learn more! 
As you do the simulator, click on objects. There might be hidden information!
OK
Bioengineer Banana Fragrance
Today, most fragrances are made using industrial chemistry. This means lots of heat, energy, and in many cases, a large impact on the environment and the health of people creating them.
We need more Banana Fragrance !
Bioengineering is set to help individuals and industries make flavours, scents and cosmetic compound sustainably, from new sources. What if there could be a safer, better fragrance without harming any plants?! Its your job to genetically engineer E. coli bacteria to produce a banana smell synthetically. In this exercise you will get a tube of DNA from your friend. This DNA is a program she developed so that when you insert it into bacteria, it will produce a sustainable fragrance!
LB is food for bacteria. Agar makes the LB jelly-like so you grow bacteria on the surface of it. LB contains sugar (glucose), tryptone (amino acids), and yeast extracts.
Pour the LB powder into the boiling water
Got it
The challenge to promote genetic engineering know-how
1. Have fun figuring out how to transition between steps. It is not always the same !  2. Click on objects throughout, there may be hidden information 3. Tweet any technical questions at @aminobiolab and @SystemsSally with #100Kbio 4. Don’t forget to download or print your Virtual Bioengineer Certificates!
-Richard Feynman, Nobel Laureate
What I cannot create, I do not understand
Great Find! Good clickin’. Fun Fact   Did you know that, despite popular belief, radioactive materials don’t bubble and glow green! We may have The Simpsons to thank for this widespread myth!
Oh! okay!
OK, I’ll Still Try
The mobile version is not ready, but you can still try the first few steps!