Confluent cells in medium with 10% fetal calf serum were wounded by a scratch with a pipette tip, and invasion of cells was monitored continuously by video microscopy.
MDA-MB-435 cells were grown to 100% confluence in a six-well plate and a scratch was created using a sterile tip.


Migrating cells within the scratch were quantified after 3 h (n=3 individual experiments, expressed relative to MDA-Pa cells).





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