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Restriction enzyme: An enzyme from bacteria that can recognize specific base sequences in DNA and cut the DNA at that site (the restriction site). Refresh kit components, reduce packaging waste, reuse components, and refresh your kits, and you’ll save storage space by purchasing individual items.
Large Class Preparation Guide Learn tips and techniques for preparing agar plates and agarose gels in large quantities.
Download the complete Biotechnology Explorer™ Refresh Kit Components Purchasing Guide. Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes.
If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number. If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the list price.
After copying the DNA from fetal amniocytes using PCR, the amplified DNA is exposed to the restriction enzyme. Unless otherwise noted, individuals pictured are models and are used for illustrative purposes only. This site is for educational purposes only and medical decisions should not be based solely on its content. Bio-Rad now has many individual components for Biotechnology Explorer™ kits available for purchase. It is vital in the fields of molecular cloning and genomic sequencing since it can be used to subclone very long genomic DNA fragments much more efficiently than plasmid vectors.

Each restriction enzyme used in this kit will cut the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes.
With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda bacteriophage.
Each restriction enzyme used in this kit cuts the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes.
To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. DNA is cut by combining it with a special type of enzyme, a restriction enzyme, which "recognizes" a unique DNA sequence. For example, many HbS (sickle cell anemia) DNA tests utilize an enzyme that only cuts DNA sequences of CTGAG (the restriction enzyme is called DdeI). If the HbS mutation is present, the DNA will not be cut and a single large fragment of DNA will remain, the original PCR product of 233 base pairs. This site, its authors, and its consultants do not assume liability for errors or omissions.
The website and Clinical Tools receive no support from the pharmaceutical or device manufacturing industries. Lambda DNA comes from a bacterial virus, or bacteriophage, which attacks bacteria by injecting them with its nucleic acid.
The three different sets of DNA fragments that result are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast DNA stain. By visualizing the effects of three different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.

The three different sets of DNA fragments that result from the enzymatic digestion are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast™ DNA stain. The mutation that causes HbS is a single base pair change (from A to T) at position 6 of the beta-globin chain gene. Conversely, if the mutation is not present, the restriction enzyme will cut the DNA into 2 pieces. Once inside, Lambda DNA hijacks the bacterial cellular machinery and replicates itself until the cells burst, releasing millions more bacteriophage to carry out the same infection process. Banding patterns from each sample are then compared to each other and to a DNA size standard. During digestion, the enzyme locates places along the DNA with the unique sequence and cuts the DNA at that site. Bacteriophage lambda is harmless to humans and other eukaroytic organisms, and therefore makes an excellent source of DNA for experimental study.
Students use their electrophoresis results to construct standard curves and determine the precise DNA fragment sizes generated by the different restriction enzymes.

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