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This section on cloning includes information that is a necessary basic for research laboratories, but may be required from time to time in clinical labs when something really interesting is found in the molecular lab.
Once the DNA has undergone restriction digestion, it may be used to recombine with any other piece of DNA that has the complementary ends, regardless of the source of that DNA.
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Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), Utrecht, The Netherlands. Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.


Restriction enzymes recognize specific nucleotide sequences (recognition sequences) in double-stranded DNA that are generally four five or six nucleotides long and then cut both strands of the DNA at specific locations. There are typically three ways in that the DNA can be cut; a staggered cut to leave a 5' over- hang for instance a short single-stranded region of DNA is left which has a 5' end and overhangs the end of the double-stranded DNA, a staggered cut to leave a 3'overhang, or a cut in the similar place on both strands to leave a blunt end. The new DNA molecule which has been made through joining the DNA fragments is known as a recombinant DNA molecule. Our experts are helping students in their studies and they offer instant tutoring assistance giving their best practiced knowledge and spreading their world class education services through e-Learning program. The shaded area indicates DNA fragment sizes that are PCR amplifiable and can be sequenced (see also Supplementary Figs.


For enzymes which cut in the staggered manner, the single-stranded tails are known as cohesive ends because they permit any two DNA fragments produced through the same restriction enzyme to form complementary base couples. Blunt-ended DNA molecules can also be connected together through DNA ligase but the reaction is less favorable.



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