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A blastocyst is an embryo that has developed in culture in the IVF laboratory for at least five days after egg retrieval and has divided into two different cell types.
There is now abundant evidence that transferring blastocyst embryos into the uterus five or six days after egg retrieval, results in higher implantation rates per embryo transferred compared to a day three embryo transfer. Traditionally, in an IVF cycle, embryos were transferred to the uterus on the second or third day of development and initial embryonic cell division. Previously, laboratory culture media could only sustain an embryo’s growth for about three days in vitro. La Jolla IVF is fortunate to have an embryology team that is highly skilled and a laboratory that is technologically advanced. This newer "sequential media" attempts to reproduce the natural environment of the maternal reproductive tract.
In general, a blastocyst or embryo transfer procedure is more advantageous for patients who develop a large number of eggs and embryos.
At La Jolla IVF however, many of the patients who are regarded as “poor prognosis” in terms of their ultimate success rate for IVF, choose to undergo what we term “embryo banking” cycles. Over the past several years, patients at La Jolla IVF have completed hundreds of fresh and frozen blastocyst embryos transfers. When you are getting ready to undergo IUI, you are probably already well aware that timing is everything.
When you are getting ready for IUI, you will need to take an ovulation predictor kit test every single day. Depending on your doctor, you will get different directions on what to do when you get a positive OPK test. After you go into the clinic for your IUI procedure, the concentrated sperm will be carefully and painlessly inserted into the uterus via a catheter. I was getting positive pregnancy test so for the fun of it I took an ovulation test and I did come back positive. This internet site provides information of a general nature and is designed for educational purposes only.
The surface cells are termed the trophectoderm and will eventually become the placenta and the inner cells, termed inner cell mass will become the fetus.
During the first few days, the embryo relies on the egg cell (from the mother) for all its growing nutrients. An embryo reaching the blastocyst stage has an increased chance of implantation as opposed to a day 3 embryo (all things being equal regarding a healthy uterus).
La Jolla IVF’s embryology team has moved systematically towards refining the techniques in the laboratory which now enable many of our patients to avail themselves to transferring blastocysts as opposed to the older more traditional methodology of day 3 transfers.
Research throughout the late 90?s identified that as the embryo develops, its nutritional requirements change due to metabolic factors. It requires much more time and effort to sustain the dynamic process of embryo development to blastocyst stage. These blastocyst culture conditions do not improve the health or viability of an individual embryo-sequential media does not transform a poor embryo into a good embryo, rather it allows embryos capable of sustained growth to continue growing in the culture media and reach their maximum inherent capability.


Because only some embryos are capable of developing into blastocysts, it is possible to have no embryos survive to day 5 to transfer. The optimization of a blastocyst cryopreservation program mandates a comprehensive approach. Since the egg is only viable for around 24 hours, it is imperative that the timing be perfected. These test kits are available over the counter at many drug stores, and they are inexpensive, so they are easy to obtain.
Some doctors will prefer to do the IUI the same day, and some will prefer to do the IUI the day after the surge. Clinically proven to dramatically increase your chances of conception and help you get pregnant fast from the very first use.
I took a OPK at 6dpo and it was close to positive and at 7DPO the test line was WAY darker then the control line, so I took a wondfo and it was positive! Also I did not use fmu for the hpt so maybe that had something to do with it being negative? A healthy blastocyst should hatch from its shell (zona pellucida) by the end of six days or earlier and is then ready to begin to implant within the lining of the uterus.
However, in order to survive post day three or four, the embryo must activate its own genes so that it can carry on growing and dividing.
It is believed that the improved implantation rates following a blastocyst transfer is due to selection of the "best" embryos. IVF practitioners were aware that day three embryo transfers were too early when compared to what happens in naturally conceived pregnancies. This research led to the development of different laboratory culture media for the embryo’s specific developmental stages. Our embryologists use specific media to facilitate the embryos development to the blastocyst in vitro during their first three days in culture inside the IVF laboratory.
The ability to develop embryos to the blastocyst stage allows our embryology team to have greater certainty about which embryos are more likely to implant.
The embryos that are frozen will be thawed and in combination with the fresh embryos and all the resulting embryos may undergo PGD (not mandatory) on day 3 of in vitro culture (see section on Preimplantation Genetic Diagnosis (PGD) for full explanation). An appropriate embryo culture system with attention to the protein supplement and a low oxygen environment has been shown to increase the number of blastocysts available for cryopreservation, improve the quality of the inner cell mass and blastocysts post-thaw survival rate. In our egg donation and surrogacy cases, the ongoing pregnancy rate is over 80% per transfer using blastocysts. During the menstrual cycle, the surge of the hormone LH is what indicates that the release of the egg is imminent. The preferred way to test is once in the morning and once in the evening, but sometimes, your doctor will tell you to only test once during the middle of the day.
Some doctors tell their patients that if they get a positive OPK in the morning or at noon, then they will do the IUI procedure the same day, but if they get the positive result in the evening, you will have to wait and do the IUI procedure the following day. IUI is a relatively simple procedure, and doesn’t require anesthesia or any other drugs.


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My period is due Thursday and I took an hpt and got bfn but I have some leftover ovulation tests so out of curiosity tried them because I have heard of people getting a positive when pregnant . Unfortunately, not all embryos are able to grow past this milestone and in fact only about one third of embryos are capable of becoming blastocysts. In a naturally conceived pregnancy, the embryo reaches the uterus on day four or five because it grows and divides in the fallopian tube for several days after fertilization. These blastocyst culture conditions do not improve the health or viability of an individual embryo, i.e. The question as to whether the embryos that did not grow into blastocysts could still have implanted if they were transferred on day 3, is not able to be definitively answered at this time.
One of the major limiting factors with doing well with this type of treatment (besides the normal versus abnormal results of the PGD) is the ability of the IVF laboratory to sustain the embryos’ growth in the incubators while PGD is being performed and to enable those embryos that are inherently capable to continue to grow to blastocysts.
These results are being achieved in our program by us routinely transferring a maximum of two blastocyst embryos. When the LH surge occurs, it usually means that ovulation will soon follow, around 36 to 40 hours after wards. Still other doctors have different procedures and protocols for their clinic’s IUI procedures. They appear to me as positive so could this mean I am pregnant but it's just not showing up on hpt yet?
If an embryo can propel itself into becoming a blastocyst in vitro then it would appear that nature has sent us a message that these embryos are the product of a "survival of the fittest" test. Barry Behr, our Laboratory Director developed one of the first media to successfully grow embryos to the blastocyst stage.
In patients with few embryos on day 3 or where no embryo selection is needed, it is still a rational approach to perform a day 3 transfer (if the patient wishes to do a transfer on their first fresh cycle) as opposed to trying to grow these few embryos to blastocysts and then potentially having no embryos to transfer.
This option has dramatically lowered the risk of patients having to deal with the stress, adversity and dilemma of carrying higher order multiples (triplets or more).
When your LH surge occurs, the Ovulation Predictor Kit will show a color that indicates that the surge has occurred, and then you will know that ovulation is getting ready to happen.
Ask your doctor so you know exactly what is going to happen after you get that positive OPK result.
If you haven’t given IUI a thought before, you should think about it if you are struggling with infertility.
Lee is Lab Director for ConceiveEasy and is board certified as a High Complexity Laboratory Director (HCLD). Our embryology team is constantly upgrading both laboratory equipment and their expertise and we are confident that these advances will ultimately translate into even higher pregnancy rates for patients.



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