FastQCFastQC Report
Fri 21 May 2021
4DNFIU52HOPQ.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFIU52HOPQ.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125308904
Sequences flagged as poor quality0
Sequence length132
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8786300.7011712431863582Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6417550.5121383872290511No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3836190.30613866034611553Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1656670.13220688611241863Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1345730.10739300696461283Clontech SMART CDS Primer II A (100% over 21bp)

[OK]Adapter Content

Adapter graph