FastQCFastQC Report
Tue 14 Jan 2025
4DNFIT86AUCS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFIT86AUCS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75795858
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGGGTATAGTGAGAACCA2696410.35574635226109586No Hit
CAGACGCGCGGGAACCTCCTTAAA2278380.30059426202418604No Hit
CAGACGCGGAATGCATGTTCAAGC2133500.28147976107084904No Hit
CAGACGCGTTTGTGCACACTTAGG2125400.280411101092094No Hit
CAGACGCGTAACCGTTGCACACTA1961240.2587529255226585No Hit
CAGACGCGTCCCTTTGAATAGTCA1845360.24346449115992594No Hit
CAGACGCGAGAATCGTGGTGCTGT1642130.21665168035963125No Hit
CAGACGCGGGGAGGACTCTTGAAT1540810.20328419529204353No Hit
CAGACGCGAGAATCGTGCCGGAAA1386780.18296250436270542No Hit
CAGACGCGCGATATAACTTATCGC1326070.17495283185527105No Hit
CAGACGCGCTGAGCAACAAAGGTA1222670.16131092545980547No Hit
CAGACGCGCAGTAATACGCCACTT1062780.14021610521250383No Hit
CAGACGCGCAGGATCACCGCCCAT1033560.13636101328914307No Hit
CAGACGCGTACCTCCTGCGGATAG992800.13098341072938313No Hit
CAGACGCGTACAACAACCTTTGAC970200.12800171745532585No Hit
CAGACGCGAATGAGCTGGCCGGAA961800.12689347747735766No Hit
CAGACGCGGCTCAGGACTCACTGT943830.12452263552449No Hit
CAGACGCGAGCCAAATGCAGGCAA883650.11658288768233219No Hit
GGGGGGGGGGGGGGGGGGGGGGGG872900.11516460437719434No Hit
CAGACGCGCAGGATCACTAAGCAT866060.11426218039513453No Hit
CAGACGCGCTTGAATCTTCCAGCA782810.1032787306134855No Hit
CAGACGCGGGGCTATGAGACTCAG769120.10147256331605878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)