FastQCFastQC Report
Tue 14 Jan 2025
4DNFIPKB6TDT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFIPKB6TDT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28148106
Sequences flagged as poor quality0
Sequence length24
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGTGCAAGGTGGCCAATC582030.20677412540651935No Hit
CAGACGCGCCGGTTCACCCGGAAT574960.20426241111924193No Hit
CAGACGCGAGGCGGTTGAGGAAGG549040.1950539762781908No Hit
CAGACGCGGCTTGTGTGTTCATTC538520.19131660226091232No Hit
CAGACGCGTCAGCGGGAGTAATCA489810.1740117079280574No Hit
CAGACGCGATAGATGCTCAATGAG401790.14274139794698798No Hit
CAGACGCGTTTGCGGCTAAGGTCG380540.13519204453756142No Hit
CAGACGCGTGCTAATCTTAATTCG364730.12957532560094806No Hit
CAGACGCGGAGGTGGGACTTAGAG362900.12892519304851274No Hit
CAGACGCGACCAAATGAGTTTGTC359110.1275787436639609No Hit
CAGACGCGAGAGCAAGAGCGCTAA357720.12708492713506195No Hit
CAGACGCGTCAGCGGGAAGTGAGA356880.12678650563558344No Hit
CAGACGCGTCTAGCGCTTAAGGAC355290.1262216363687134No Hit
CAGACGCGATTTGCGCTGTGGGTA345650.12279689439850766No Hit
CAGACGCGGGAGCAAACGCGAACC337570.1199263637844763No Hit
CAGACGCGTAGTCAAACTATGGAT316310.11237345773815119No Hit
CAGACGCGATAGCCGGACCAGGGT314670.1117908252867884No Hit
CAGACGCGTGCTAGCCTAGGCTTT301520.10711910776519032No Hit
CAGACGCGTTTGCGGCTCTATTGA301060.10695568646785684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)