FastQCFastQC Report
Tue 14 Jan 2025
4DNFIO5TW475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFIO5TW475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72986283
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGGGGAACCTGTAATGGA1868360.2559878271921314No Hit
CAGACGCGCTAACAGGATTATTGC1719800.2356333175646169No Hit
CAGACGCGCCAATATCTTCAACCG982620.13463077712835436No Hit
CAGACGCGCGGTTAGACTCAATAG971900.13316200798991232No Hit
CAGACGCGGGAACAAGAGCTTGCC931050.1275650658905318No Hit
CAGACGCGCTCTAGCGACGAAGGA866780.11875930166220412No Hit
CAGACGCGAAATGACCTCACAACC861360.11801669637019328No Hit
CAGACGCGCATAGTGGATTACGGA858700.1176522443265127No Hit
CAGACGCGCGATCCGTGTTCAAGC818100.1120895552387563No Hit
CAGACGCGCGTAAATACCTTTACG817260.11197446511969927No Hit
CAGACGCGCAATGCGACCTCACAT795150.10894512877166249No Hit
GGGGGGGGGGGGGGGGGGGGGGGG743460.10186297608826031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)