FastQCFastQC Report
Tue 14 Jan 2025
4DNFILLW25IU.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFILLW25IU.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14266664
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGAGTGAATCTCCTAATA539260.37798605196000973No Hit
CAGACGCGACTGACAACGATTGCA378770.26549304027907294No Hit
CAGACGCGGGCAGGTTGGCAAATG362950.25440425315967347No Hit
CAGACGCGACTGAGCGATTAGCTT325380.2280701360878759No Hit
CAGACGCGGTACCTCACGCGTTTC317980.22288321923050824No Hit
CAGACGCGATAGATGCTGATTTGC307510.21554443281204352No Hit
CAGACGCGTTGCAGGACGTAACGT304130.21317527349070534No Hit
CAGACGCGATAGATGCTGTACGTT300880.2108972356817263No Hit
CAGACGCGGGTTAACACGCCCAGA299380.20984583361604367No Hit
CAGACGCGCTATTAGCTTAAGCCC297590.20859116048432907No Hit
CAGACGCGCGGTATTTGATATTCG293890.20599770205564524No Hit
CAGACGCGGGCTAGCACTAGTCTC283640.1988131212734806No Hit
CAGACGCGAAGCTTAGAACAGGCT273220.1915093815905386No Hit
CAGACGCGGCTTTGTGAATAATGC272970.19133414791292486No Hit
CAGACGCGTAGCGGATGCCTCCTA269490.18889489512054114No Hit
CAGACGCGAAGATAGACCTTGCTA266840.18703741813783517No Hit
CAGACGCGAACGGGAACCTTACCA261930.1835958287095007No Hit
CAGACGCGTCCCTCATGACTCACG256380.17970564106647496No Hit
CAGACGCGCGCACCATGCACTTGA253340.1775747995466915No Hit
CAGACGCGGGTTAACACCACAAAT252940.17729442566250947No Hit
CAGACGCGTGACAAATGATTACGT251070.17598367775395846No Hit
CAGACGCGAAGCCTAACGGGCGTT250540.17561218235741727No Hit
CAGACGCGGGTTTATTGGTTCAAT244700.17151872364835957No Hit
CAGACGCGTGTTGTTGACATAACG240640.1686729287239119No Hit
CAGACGCGTCATGAGGATGTAACG235060.16476171303957254No Hit
CAGACGCGCTCAAAGGAGGTTGGT230390.1614883479417473No Hit
CAGACGCGTCCTAGTACGGTTTAG223840.15689722558826646No Hit
CAGACGCGGGTTGGTGAAGTTATG220930.15485750558084216No Hit
CAGACGCGTTATAGCCTACCTCCT214860.1506028318883798No Hit
CAGACGCGGCTCAATCTTGAAGTG213050.1493341400624561No Hit
CAGACGCGCGCCAACTGCGATTAC211270.14808647627784605No Hit
CAGACGCGCGGTTAGACTTCCTCC211170.14801638280680052No Hit
CAGACGCGTGTTGGCCTCAATCTA208700.14628507407197644No Hit
CAGACGCGTAACGTGGAAGTCAGC206940.14505142898157553No Hit
CAGACGCGGTGAACCGATCCCTTT206700.1448832046510663No Hit
CAGACGCGCCATTATGATTACGGA206520.14475703640318438No Hit
CAGACGCGAGACTAGTGCAGGCAA205370.14395096148616102No Hit
CAGACGCGCAATGAGCTTGACTTA204820.14356544739541072No Hit
CAGACGCGCTGACTATGAAACTGG204520.1433551669822742No Hit
CAGACGCGCCTAAAGGACGAATAC203050.14232479295790523No Hit
CAGACGCGGCTGAATACGCTTGAA202300.14179909192506393No Hit
CAGACGCGGCACCTTACCGGCTAA201910.14152572738798644No Hit
CAGACGCGCCTTTATTGGACATAG201720.14139254979299998No Hit
CAGACGCGGGTGTGACTACTTCGA199660.1399486242894625No Hit
CAGACGCGTAGCTTACTCAATCTA199280.13968226909948955No Hit
CAGACGCGGTCCGGAACTTGGATA198220.13893927830640718No Hit
CAGACGCGTTGAGCTTGAGCAAGT196250.13755843692681063No Hit
CAGACGCGGCTGCAAGACCGAAGC194330.13621264228273688No Hit
CAGACGCGTGCTTATGATCACAGA194130.13607245534064588No Hit
CAGACGCGGGAATATCTTTAACCA192530.13495095980391772No Hit
CAGACGCGCAGATAATGATCCTAA192350.1348247915560358No Hit
CAGACGCGTGTTGGTTGCTCAAAC191280.13407479141584888No Hit
CAGACGCGGGTTAGTACGTCCAGG187880.13169161340030158No Hit
CAGACGCGTCAGCAAGAAGTGAGA187830.13165656666477882No Hit
CAGACGCGATAACCGACAGGCTTA187540.13145329559874686No Hit
CAGACGCGGCTTCATGACCGTTAC187090.13113787497904206No Hit
CAGACGCGTCGATTCACTCAATGA186250.13054908982225977No Hit
CAGACGCGGCTTATCGATACTTAC184930.12962385600445908No Hit
CAGACGCGGGTTCACTGCATGAGG183950.12893693998821307No Hit
CAGACGCGTCGTTAACTTGAACAG182000.12757011730282566No Hit
CAGACGCGCTAGGCGGAACTAAAC180530.12653974327845668No Hit
CAGACGCGCCCGAAGGAAGGACAA180250.12634348155952926No Hit
CAGACGCGGTGTTTGCTTCAATAC179290.1256705842374924No Hit
CAGACGCGAAATGCTGATTAGTGG178900.1253972197004149No Hit
CAGACGCGCCTTGTTCTTAAGGGT178860.12536918231199667No Hit
CAGACGCGTCGCAACCTAGATTCC177200.12420563069264125No Hit
CAGACGCGCCGGTTTGAGAGGCTT175590.12307712580880857No Hit
CAGACGCGGATTGCGGACCTCATG172920.12120563013189349No Hit
CAGACGCGACTTAGGACTTAAGGT171860.12046263933881109No Hit
CAGACGCGTGTCCTTCTAACGGTG171580.12026637761988367No Hit
CAGACGCGGGAGCTTGACATAGAC171570.12025936827277912No Hit
CAGACGCGCACTGGCTGACCGTTG171210.12000703177701527No Hit
CAGACGCGCATAGCTTGGCCATCA170490.11950235878548762No Hit
CAGACGCGGCATAGCGAACCAGCA169680.118934601670019No Hit
CAGACGCGCTTGAATCTCAGCTCA169410.11874534929819612No Hit
CAGACGCGATATCAGGATCGATTT169070.1185070314966414No Hit
CAGACGCGTTAAGGTACGCTTAAG168340.11799534915800919No Hit
CAGACGCGCCGTGAGGAACAGTAT167830.1176378724556771No Hit
CAGACGCGTGTGGACACCCTCAAT167790.11760983506725889No Hit
CAGACGCGGCTCATTGATGGCCGA167150.11716123685256764No Hit
CAGACGCGTAGTCTCACAATTGGC166690.1168388068857583No Hit
CAGACGCGGCGGTCAACGGGCGTT165160.11576637677876202No Hit
CAGACGCGGGGTGAAACGATTGCA163440.11456076907677927No Hit
CAGACGCGCTATTGTGAGGGTGGA161720.11335516137479652No Hit
CAGACGCGAGGCCTTTGTTAAGCC161310.11306777814350995No Hit
CAGACGCGCATTTAGCTAGATTCC161220.11300469401956897No Hit
CAGACGCGACGGTGTGACTCAATT158940.11140656287973137No Hit
CAGACGCGGTGAAGCCTTCACCAG158240.11091590858241282No Hit
CAGACGCGCGCAGGACTCATAGCC157260.11022899256616685No Hit
CAGACGCGGATGAAGGACATAACG156920.1099906747646121No Hit
CAGACGCGGCGGTGTACGTAGCTA155190.1087780577155248No Hit
CAGACGCGTGATTTGCTTTAACCA155160.10875702967421115No Hit
CAGACGCGAATAAGCCTTATTACG151940.10650001990654577No Hit
CAGACGCGCCGTGAGGACCTAGCT151590.1062546927578865No Hit
CAGACGCGGTGCACTTGAGCAAGT151380.10610749646869093No Hit
CAGACGCGGCTATGTCTTAGGAGT151300.10605142169185452No Hit
CAGACGCGTGCACTTACTTAAAGC151280.10603740299764543No Hit
CAGACGCGGCCTCAATGTAACGAA150750.10566590760110423No Hit
CAGACGCGCGCCAACTGTTTAGCG149370.1046986177006762No Hit
CAGACGCGAATCACAGAGGTCCAA149170.1045584307585852No Hit
CAGACGCGGTGAAGCCTATCGCAT147580.1034439445689616No Hit
CAGACGCGTGACAACGATTAGGAC147440.1033458137094979No Hit
CAGACGCGTGTGATTCTCACTTGG147320.10326170154424327No Hit
CAGACGCGAACCAGGCTAGGCTGA146900.10296730896585216No Hit
CAGACGCGGCGGTAACTTCACCAG146750.10286216875928388No Hit
CAGACGCGTGCCAGAACGTGCTTC146240.10250469205695178No Hit
CAGACGCGATATGCGACCTTTAGC145460.10195796298279683No Hit
CAGACGCGAAAGGACGACCGTTAC144930.10158646758625563No Hit
CAGACGCGCATAGTGGATCCTAAT144470.10126403761944629No Hit
CAGACGCGGTGTAACACCTTTAGC143850.10082945809896413No Hit
CAGACGCGACGGATCGACCTAGCT143430.10053506552057298No Hit
CAGACGCGGTGAGGAACTGATGAT143220.10038786923137742No Hit
CAGACGCGCTTGTTCCTTGTCCAG143050.10026871033060006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)