FastQCFastQC Report
Tue 14 Jan 2025
4DNFIBTC74LL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFIBTC74LL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80219634
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGGGTATAGTGAGAACCA2688410.33513117250073715No Hit
CAGACGCGCGGGAACCTCCTTAAA2296420.28626657658398197No Hit
CAGACGCGTTTGTGCACACTTAGG2171110.27064571249477404No Hit
CAGACGCGGAATGCATGTTCAAGC2134170.26604085478624845No Hit
CAGACGCGTAACCGTTGCACACTA1933150.24098215157650807No Hit
CAGACGCGTCCCTTTGAATAGTCA1812330.22592100083627908No Hit
CAGACGCGAGAATCGTGGTGCTGT1636930.20405602947527784No Hit
CAGACGCGGGGAGGACTCTTGAAT1555850.19394877817567704No Hit
CAGACGCGAGAATCGTGCCGGAAA1441270.17966549186699107No Hit
CAGACGCGCGATATAACTTATCGC1317230.16420294313484401No Hit
CAGACGCGCTGAGCAACAAAGGTA1238370.15437243206569604No Hit
CAGACGCGCAGGATCACCGCCCAT1067460.13306717405367371No Hit
CAGACGCGCAGTAATACGCCACTT1051090.13102652649848787No Hit
CAGACGCGTACCTCCTGCGGATAG986520.12297737484067803No Hit
CAGACGCGTACAACAACCTTTGAC974620.1214939474792418No Hit
CAGACGCGGCTCAGGACTCACTGT947890.11816184551527623No Hit
CAGACGCGAATGAGCTGGCCGGAA935730.11664600713585903No Hit
CAGACGCGAGCCAAATGCAGGCAA901480.11237647880567495No Hit
CAGACGCGCAGGATCACTAAGCAT871370.10862303360795687No Hit
CAGACGCGCTTGAATCTTCCAGCA814460.10152876040297068No Hit
CAGACGCGGGGCTATGAGACTCAG803030.10010392218942311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)