FastQCFastQC Report
Fri 8 Aug 2025
4DNFIA9HYCDV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFIA9HYCDV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44904854
Sequences flagged as poor quality0
Sequence length35-37
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12635722.813887336099567No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG468160.10425598978676114TruSeq Adapter, Index 20 (100% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)