FastQCFastQC Report
Tue 14 Jan 2025
4DNFI93ONUYS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFI93ONUYS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78350113
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGGGTATAGTGAGAACCA2819200.35982079566368974No Hit
CAGACGCGCGGGAACCTCCTTAAA2403150.306719404476162No Hit
CAGACGCGGAATGCATGTTCAAGC2270900.28984004145597086No Hit
CAGACGCGTTTGTGCACACTTAGG2211070.28220380486241287No Hit
CAGACGCGTAACCGTTGCACACTA2045190.26103216979406274No Hit
CAGACGCGTCCCTTTGAATAGTCA1933290.24675012274711078No Hit
CAGACGCGAGAATCGTGGTGCTGT1733460.22124537331554328No Hit
CAGACGCGGGGAGGACTCTTGAAT1586420.20247832954625095No Hit
CAGACGCGAGAATCGTGCCGGAAA1428590.1823341339660863No Hit
CAGACGCGCGATATAACTTATCGC1401200.1788382870615643No Hit
CAGACGCGCTGAGCAACAAAGGTA1253360.15996913750462619No Hit
CAGACGCGCAGTAATACGCCACTT1113150.14207382189735962No Hit
CAGACGCGCAGGATCACCGCCCAT1071950.1368153738336025No Hit
CAGACGCGTACCTCCTGCGGATAG1042180.13301576221083433No Hit
CAGACGCGTACAACAACCTTTGAC1023300.13060606562239419No Hit
CAGACGCGAATGAGCTGGCCGGAA1012940.1292837956723815No Hit
CAGACGCGGCTCAGGACTCACTGT967540.1234892922234841No Hit
CAGACGCGAGCCAAATGCAGGCAA911570.11634571605531699No Hit
CAGACGCGCAGGATCACTAAGCAT888380.11338592453593525No Hit
GGGGGGGGGGGGGGGGGGGGGGGG849490.1084223069340053No Hit
CAGACGCGCTTGAATCTTCCAGCA800860.10221555136748814No Hit
CAGACGCGGGGCTATGAGACTCAG799030.10198198437824843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)