FastQCFastQC Report
Fri 8 Aug 2025
4DNFI5SDDO9F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFI5SDDO9F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67266131
Sequences flagged as poor quality0
Sequence length35-37
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18407842.7365688685142304No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAC3464450.5150363115131447TruSeq Adapter, Index 5 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)