FastQCFastQC Report
Tue 14 Jan 2025
4DNFI4MUQN5P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFI4MUQN5P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165058536
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT7122810.43153236255530586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6624490.40134186092623525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4275880.25905234007406924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT2331200.1412347435336516Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)