FastQCFastQC Report
Fri 8 Aug 2025
4DNFI463CFPO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFI463CFPO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67559409
Sequences flagged as poor quality0
Sequence length35-37
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19369452.867024784068197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT1305380.19321957064485273TruSeq Adapter, Index 15 (100% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)