FastQCFastQC Report
Tue 14 Jan 2025
4DNFI2B4XXZA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFI2B4XXZA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12992176
Sequences flagged as poor quality0
Sequence length73
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG548370.42207710240378515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA280700.21605310765494554Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT137570.10588680448910175Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)