FastQCFastQC Report
Tue 14 Jan 2025
4DNFI22ECE32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4DNFI22ECE32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76722107
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGGGTATAGTGAGAACCA2530740.32985798995327387No Hit
CAGACGCGCGGGAACCTCCTTAAA2121980.27657999538516326No Hit
CAGACGCGTTTGTGCACACTTAGG2081090.2712503711609484No Hit
CAGACGCGGAATGCATGTTCAAGC1978960.25793869295065114No Hit
CAGACGCGTAACCGTTGCACACTA1814860.23654981216821902No Hit
CAGACGCGTCCCTTTGAATAGTCA1702480.2219021435373249No Hit
CAGACGCGAGAATCGTGGTGCTGT1519000.1979872633059986No Hit
CAGACGCGGGGAGGACTCTTGAAT1495000.1948590906138696No Hit
CAGACGCGAGAATCGTGCCGGAAA1381200.18002633843202456No Hit
CAGACGCGCGATATAACTTATCGC1212850.15808351040202795No Hit
CAGACGCGCTGAGCAACAAAGGTA1176780.1533821275268157No Hit
CAGACGCGCAGGATCACCGCCCAT1013940.13215747581072038No Hit
CAGACGCGCAGTAATACGCCACTT969460.1263599290879746No Hit
CAGACGCGTACCTCCTGCGGATAG927930.12094688692530302No Hit
CAGACGCGGCTCAGGACTCACTGT918200.11967867357970241No Hit
CAGACGCGTACAACAACCTTTGAC917360.11956918753547788No Hit
CAGACGCGAATGAGCTGGCCGGAA880790.1148026343958463No Hit
CAGACGCGAGCCAAATGCAGGCAA857200.11172790132054115No Hit
CAGACGCGCAGGATCACTAAGCAT831970.10843940977794053No Hit
CAGACGCGCTTGAATCTTCCAGCA774530.10095264980144511No Hit
CAGACGCGGGGCTATGAGACTCAG770060.10037002763753607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)