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We tested the accuracy of the Reflotron System by comparing results given by this system for esti-mations of glucose, SGOT, SGPT, cholesterol and triglycerides on patients samples which were already analysed on either an autoanalyser HITACHI 705 or manually on CLIN ICON 4010. The Reflotron System is designed for the quantitative determination of clinical chemistry parameters on whole blood, serum or plasma. The success of reagent strips for moni­toring of clinical chemistry parameters in blood and urine1-2 and the easy workability and accuracy of reflectance photometers for their evaluation3-4 have extended the use of this so called “ dry chemistry” reagent system to analyse a number of parameters in blood. Routine patients serum samples analysed on autoanalysers Hitachi 705 and Clinicon 4010 were also tested on the Reflotron system for comparative analysis. Figure I shows the comparison of results of glucose estimation on Reflotron with Hitachi 705. Figure 6 shows comparison of Reflotron cholesterol estimation c whole blood vs that on serum of the same samp1.
Figure 7 shows Reflotron results of SGPT estimation on EDTA whole blood vs that on serum of the same sample. Reproducibility of the Reflotron system was checked by 10 consecutive estimations of glucose en EDTA whole blood and SGPT on serum of one patient as shown in Table. Our experience with similar system ror glucose estimation has been very encouraging6 and the present paper highlights the importance of reagent strips and their portable analysers.
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Reflotron estimations correlated well with both Hitachi and Clinicon estimations and the strips gave reproducible results. This system consists of a Reflotron: a compact portable reflectance photometer for fully automa­tic evaluation of Reflotron Tests.


Since these reagent carriers have a long shelf life5 and the photometers are small portable equipments, both mains and battery operated, this makes them ideally suited for small volume laboratories, health care centres, physician clinics etc.
The number of serum samples analysed both on Hitachi and Reflotron were glucose 37, SGOT 43 and SGPT 47. It is a modern automated analysis system that takes over both manual procedures and the calculations from the operator. The Reflotron System7 is capable of estimating most routine chemistries while other systems have increased the scope of such systems even further by incorporating therapeutic drugs on reagent strips8.
Ejazuddin and Com­pany for providing the Reflotron System and all the accessories for making this study possible. We for small laboratories in our expanding rural health conclude that this or similar systems will be ideal services (JPMA 38: 205, 1988). Within the Reflotron a micro-processor system controls all functions such as temperature regulation, automatic calibration, specific test procedure, evaluation and calculation of results. Their use will be of special value in Pakistani set-up at village and tehsil levels where infrastructure for full-scale laboratories is neither available nor financially possible. The number of serum samples analysed both on Clinicon and Reflotron were Cholesterol 43 and Triglycerides 44. In fact only sampling remains, while the machine does the rest, ending in a display of the result. We conclude that the Reflotron System gives comparable results to standard lab methods and thus this system is ideal for small laboratories wanting to do large numbers and types of tests but with small workloads. Reflotron Strips are strip reagent carriers designed for specific determination of important clinical chemi­stry parameters directly on undiluted whole blood samples. This paper presents the quality assessment of one of such systems available in Pakistan, the Reflotron System. In 16 patients for SGPT and 15 for cholesterol both EDTA anticoagulated whole blood and serum samples were analysed on Re. SGOT: using alphaketoglutarate and alanine sulphinate as substrates coupled with pyruvate oxidase and peroxidase via an indicator.


We conducted this trial for checking the accuracy of this system as compared to standard autoanalyser and manual methods. This is possible due to the incorporation of a plasma separating system on the Reflotron Strips. The levels of cholesterol, triglycerides, glucose, SGOT and SGPT were evaluated on the Reflotron System on patients samples after they had been assessed on either a autoanalyser (Hitachi 705) or manually on (Clinicon 4010).
The Reflotron System gives comparable results to these standard lab methods in the few chemistries tested both on whole blood and serum. The accuracy and reproducibility of this system is presented, as compared to stan­dard laboratory methods, the object being the possible use of such systems in our rural health care centres. Reproducibility of the Reflotron System was checked by 10 consecutive analyses of SGPT on serum and of glucose on EDTA whole blood on one sample. The methodologies used for analysis on Hitachi 705 were: glucose- GOD-PAP using 4-amino-phenazone as substrate, SGOT- according to IFCC using Alpha Oxoglutarate and aspartate as substrates coupled with malate and lactate dehydrogenase, SGPT-according to IFCC using alpha-oxoglutarate and alanine as substrates coupled with lactate dehydxogenase. The quality of tests done on Hitachi and Clinicon were controlled by commercial sera with known levels of constituents in the abnormal (PRECIPATH) and normal (PRECINORM) ranges from Boehringer.
The metho­dologies used for analysis on Clinicon 4010 were, Cholesterol-CHOD-PAP using cholesterol esterase coupled with cholesterol oxidase and peroxidase with 4.aminoantipyrine as colour reagent.
Triglycerides were estimated by GPO-PAP using lipases for hydrolysis coupled with glycerol kinase, glycerol -3-phosphate oxidase and peroxidase and 4-aminoantipyrine as colour reagent. Reagent systems for Hitachi in kit form were supplied by (Boehringer) and for Clinicon 4010 by (Human). Correlation coef­ficient and regression equations were calculated for each of these comparisons. Similarly correla­tion coefficient and regression equation was calculated for Reflotron estimation of SGPT and glucose on EDTA whole blood vs serum.



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