04.07.2014
The CAMAG Automated Multiple Development procedure allows thin-layer chromatography to be utilized for tasks that could not be performed by TLC in the past. The CAMAG BioLuminizer® is a detection system specifically designed to detect bioluminescence on HPTLC plates.
For proper execution of the dipping technique, the chromatogram must be immersed and withdrawn at a controlled uniform speed. The Drying Rack consists of ten individual aluminum trays, 20 x 20 cm, which can be stacked quickly and conveniently. The CAMAG Flat Bottom chamber permits the chromatogram to be developed under conditions of partial or complete saturation of the tank atmosphere with solvent vapors. In the CAMAG Horizontal Developing Chamber, a plate can be developed in the sandwich as well as in the tank configuration. The CAMAG Nanomat 4 serves for easy application of samples in the form of spots onto TLC and HPTLC layers, precisely positioned and without damage to the layer.
Test dye mixtures are useful for functional checks on individual steps in the TLC procedure and for studying the influence of specific parameters. The CAMAG TLC Plate Heater is designed for heating TLC plates to a selected temperature after a staining reagent has been applied. The CAMAG TLC Spray Cabinet ensures the complete removal of reagent mist while spraying TLC plates. The UV Cabinet 4 is designed for inspecting thin-layer chromatograms or other objects under UV light in absence of ambient light. The easy to navigate user interface effectively guides the user through the chromatographic process – from definition of samples and substances to reporting of results. With visionCATS relevant samples can be located easier and faster than ever: a powerful search tool within the file explorer that includes extended preview functionalities enables the user to easily search for text and date, samples, methods, and analysis files.
Based on the sample oriented approach pursued with visionCATS, it is possible to compare profiles of samples developed in different analyses. Image Enhancement ToolsvisionCATS supports low-noise, high-dynamic-range imaging (HDRI) and includes a comprehensive set of Image Enhancement Tools. Scanning Densitometry and Spectral EvaluationFollowing their chromatographic separation substances on the plate can be measured by Scanning Densitometry. Quantitative AnalysisTo determine the substance concentration in a sample after densitometric or image analysis, five different quantification functions (e.g. HPTLC Method LibraryFor seamless import of validated methods and images of standards and reference materials, visionCATS provides a free of charge HPTLC Method Library for licensed users.
Controlled by visionCATS, the CAMAG TLC Visualizer can generate low-noise, high-dynamic-range images (HDRI). The comprehensive set of Image Enhancement Tools in the Data View exploits the TLC Visualizer’s full potential. For analysts using HPTLC for identification of herbal drugs or analysis of food samples, visionCATS offers breakthrough support with the HPTLC Method Library. An Image Comparison Viewer file with reference images against which each analyzed sample can be compared and evaluated, based on acceptance criteria specified in the method document. The HPTLC Method Library contains methods included in the USP Dietary Supplement Compendium and those published by the International Association for the Advancement of HPTLC. 21 CFR Part 11 The option 21 CFR Part 11 supports customers to work in a controlled environment and to establish regulatory-compliant processes.


System Suitability Test (SST) System Suitability Tests are used on each plate to check and ensure that the analysis was performed appropriately to produce results that are within the acceptance criteria established during method development.
Data Security Data Security is achieved through access control, which is provided by a flexible, secure, and customizable User Management System and basic system settings. Data Safety The visionCATS Backup Assistant helps users to store their data into a backup file. 1x 1394 FireWire® (4-pin input with external power adapter and 6-pin output) for each TLC Visualizer connected. As complexes algorithms are computed on the graphic card, a middle range model will increase speed over an entry level card.
At installation the PC should have the final name and should be in the finally used domain (name change of PC after installation is not supported). The following list contains the more common types of precoated plates and sheets manufactured by E.
The Chromimage® Documentation scanner is a flatbed scanner equipped with a dual lamp - 254nm UV and visible lamp - and driven by a digitizing software. Maximum scanning area: 210x297mm, with customized frames of 100x100mm, 100x200mm and 200x200mm.
All accuracy (comparison with known values), precision (repeatability), linearity, and sensitivity values obtained with the Chromimage® Quantidoc for the visible and UV mode analyses are consistent with recognized validation guidelines for pharmaceutical analysis by TLC.
When it comes to work on TLC images taken with another apparatus than the Chromimage® scanner, we recommend you to use the software ChromEasy. Industries and universities could not replace thin-layer chromatography technique with any other one, for their identification and quantitative analytical issues.
Our advertising formats support you in getting your message across to a clearly defined target audience. The ATS 4 offers fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. The degree of layer pre-saturation can not be controlled unless additional accessories are used. Sample application is automatic, only changing the syringe (filling, inserting and rinsing) is manual. It comes with a rubber pump but may also be operated from a compressed air or nitrogen supply. The body with slide rails, the handle and the removable bottom are all made of stainless steel.
Not only are they more convenient, their quality is superior to that of layers prepared from the type of adsorbents available for self-coating. The software organizes the workflow of HPTLC, controls the involved CAMAG instruments, and manages data.
The modular design of winCATS allows to select or deselect any step of the procedure as appropriate for the given analytical task. Simply select one of the default methods and start working: fill in the sequence table, select a mobile phase and the derivatization reagent.
The comparison can be performed in different display modes and allows the application of integration parameters. Images are automatically captured based on an optimized control of the illumination and parameters specified in the HPTLC method.


The spectral range of light from 190 to 900 nm is available for selecting single wavelengths for Scanning Densitometry. The best fitting calibration model with single-level calibration or multilevel calibration via linear, polynomial or Michaelis-Menten regression is selected. For quantification, data from the multi-wavelength scan at the optimum wavelength for each compound is used. A new analysis with all method relevant information (reference substances, plate layout, stationary and mobile phase, etc.) can then be processed. After comparing the data of the samples with the reference images individual reports can be generated by a mouse click. This UV scanner is used to perform quality control analysis (TLC technique), or to digitize any work material that contains substances detectable only under 254nm UV (papers, electrophoresis plate, TLC plates and sheets, etc.). This combination is the coupling of the UV scanner Chromimage® Documentation with a plug-in software named Galaxie®-TLC. Actually, the ability to visualize the presence of substances on TLC plate remains a reliable and reproducible technique, with cost-effectiveness and fast treatment as a bonus! Several scanning steps and up to five different evaluations can be performed in one analysis file. Any sample that has ever been analyzed can be directly compared with any other sample using the Image Comparison Viewer. Sophisticated algorithms guarantee the highest image quality for identification of even the weakest zones.
Detection can thus be fine-tuned to match the spectral properties of the analyte to its optimized specificity and sensitivity of the detection. Chromimage® Quantidoc package offers the same functions as any chromatographic software (for e.g. The new sample-oriented approach allows for creating virtual plates from tracks originating from different plates, e.g. Samples can be rearranged and compared to reference substances or samples, independent of their plate origin.
The Data View allows visual evaluation of a plate in different illumination modes and offers a broad range of helpful tools, e.g. The selected peak positions, which are detected from a previous densitogram, are automatically scanned at the defined wavelengths.
With the powerful user management, it is possible to reflect the customers’ individual safety regulations.
Spectra can be measured from 190 to 900 nm and displayed individually or overlaid in one diagram.
Each scanning step may also include up to 31 individual multi-wavelength scans using different light sources (deuterium lamp, mercury lamp, tungsten lamp). Integration of peaks and assignment to separated substances is a matter of a few mouse clicks.



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