15.12.2015
E8000 has exceptional adhesion to wood, metal, glass, fiberglass, ceramics, masonry and concrete. Return Policy details Buyers can receive a partial refund, and keep the item(s) if they are not as described, or possess any quality issues by negotiating directly with seller. Note: Due to possible delay of exchange rate update, price in various currencies is for reference only. An optical accelerometer with a bulk-micromachined silicon proof mass and a microfiber loop resonator (MLR) sensor was developed. Shibboleth is an access management service that provides single sign-on to protected resources. AbstractNeuronal control with high temporal precision is possible with optogenetics, yet currently available methods do not enable to control independently multiple locations in the brains of freely moving animals.
For coupling the fiber with the diode, the fiber was held by a micromanipulator enabling 6 degrees of freedom (II in Fig.
It replaces the multiple user names and passwords necessary to access subscription-based content with a single user name and password that can be entered once per session. Here, we describe a diode-probe system that allows real-time and location-specific control of neuronal activity at multiple sites.
In the context of implantable arrays, diodes for efficient coupling to fibers require the following: high light intensity, low current consumption, small emitter size, and light weight. 2B); another micromanipulator (I) held the prewired diode, which was connected to a precision current source. Manipulation of neuronal activity in arbitrary spatiotemporal patterns is achieved by means of an optoelectronic array, manufactured by attaching multiple diode-fiber assemblies to high-density silicon probes or wire tetrodes and implanted into the brains of animals that are expressing light-responsive opsins. Numerous miniature diodes from various manufacturers were inspected and tested for light-coupling potential with fibers. If your institution uses Shibboleth authentication, please contact your site administrator to receive your user name and password. Each diode can be controlled separately, allowing localized light stimulation of neuronal activators and silencers in any temporal configuration and concurrent recording of the stimulated neurons. Because the only connections to the animals are via a highly flexible wire cable, unimpeded behavior is allowed for circuit monitoring and multisite perturbations in the intact brain. To minimize tissue damage, fiber diameter was narrowed, and the fiber end was morphed into a pointed tip as follows.
The diode is coupled to a 50-?m multimode optical fiber, etched to a point at the distal end.


The other ends of the wires were soldered to a SIP connector (Mill-Max), twisted, and isolated using hot glue (Fig. The diode and fiber were initially oriented such that the fiber was approximately perpendicular to the emitter of the diode, at a distance of <2 mm. The capacity of the system to generate unique neural activity patterns facilitates multisite manipulation of neural circuits in a closed-loop manner and opens the door to addressing novel questions.closed-loop controlmultichannel recordingsmulticolor stimulationoptogeneticsrecent advances in optogenetics allow expressing light-sensitive channel proteins in specific cell types in virtually any excitable tissue and animal species (Deisseroth 2011).
The number of novel opsins and solutions for cell-type specific expression has been rapidly increasing (Fenno et al. Before coupling, the light power of each wired diode was measured: when driven by a calibrated current source (20–60 mA, depending on the diode), these diodes yielded 10–15 mW of light.
Next, the sensor was positioned, and the diode micromanipulator (I) was adjusted in three dimensions to maximize the power while keeping a small gap (10–20 ?m) between the diode and the fiber end. Light intensity as a function of axial and transverse distance from the fiber tip (left) and as a function of axial distance alone (right) shown for a blue (red) light source of 40 (300) ?W. 2011), and the temporal dynamics of most opsins is within the millisecond range (Boyden et al.
A drop of UV-curable glue (NOA-61; Norland Products) was placed on the fiber-diode interface, the power was measured, and the location was reoptimized. Six assemblies (4 blue and 2 red) were attached to separate shanks of a 6-shank silicon probe. Top: magnified frontal view of all 6 shanks (top middle) and an oblique view of 2 of the shanks (top right). Several laboratories have developed solutions to deliver optical stimulation to deep brain structures while simultaneously recording neurons (Anikeeva et al. For all fibers, the proximal end was kept jacketed and hand-polished to a symmetrical convex shape (5-?m polishing paper).
Top: explant of a multidiode array after 2 mo of recordings from the right dorsal hippocampus. The diode probe, equipped with 6 light-emitting diode (LED)-fiber assemblies, was left in situ, and 3 nonadjacent shanks were illuminated while photographing the skull from the inside out. However, a number of scientific questions require manipulating neurons in vivo at multiple sites independently at high spatial and temporal resolutions. For example, testing whether and how the rules of spike timing-dependent plasticity (Bi and Poo 1998) apply to the in vivo situation would require manipulation of spike timing of discrete neurons in relation to ongoing brain patterns. Creating “synthetic” receptive fields by pairing native patterns with induced spiking would offer important insights into the mechanisms of neuronal selection (O'Keefe and Nadel 1978).


Examining the circuit and behavioral impact of spatiotemporally sequenced activity at different timescales could address the debate on rate vs. Finally, a prerequisite for sensory machine-brain interfaces is the ability to generate spatially distributed activation of distinct neuronal groups in real time.
Satisfying these requirements is especially challenging for small laboratory rodents, since increasing the weight of the headgear and tether impairs the animal's freedom of movement.Circuit analysis of neuronal networks requires simultaneous monitoring of the controlled neurons. Current techniques for large-scale recording of multiple single cells in behaving animals include wire tetrodes and silicon probes. Since such electrodes can only record and disambiguate spikes of nearby (up to 60 ?m) neurons (Buzsaki 2004), stimulation of continuously monitored cells requires placing the light source very close to the recording site. However, stimulation through light sources placed on the surface of the brain (Huber et al. 2008) or large fibers placed in the brain parenchyma a few hundred micrometers from the recording sites (Airan et al.
2010) inevitably activates many more unmonitored neurons than are being monitored, making the separation between direct and population-mediated effects impossible.
Moreover, the high intensity used for the activation of deep neurons may generate superposition of multiple spike waveforms (Royer et al. Solutions for spatially confined activation of simultaneously monitored neurons were recently described by delivering laser pump-supplied light through optic fibers positioned above the recording sites (Anikeeva et al. However, applying light at multiple brain sites independently with such approaches would require multiple external laser pumps connected to bulky and stiff fiber bundles, which constrain movement of the animal. The diode-fiber assemblies are in turn attached to individual shanks of a multishank silicon probe or to wire tetrodes. The diode-probe method permits local (shank- or tetrode-specific) photostimulation and recording of the stimulated neurons without spike waveform superposition or light artifacts. Because the method relies on solid-state light-emitting diode (LED) and laser diode technology, it allows fast multisite, multicolor optogenetic manipulations in behaving animals and concurrent monitoring of the manipulated neurons without limiting free movement of the animal.



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