28.11.2014
The modular nature of our products allows you to source at the component, sub-system, or system level, depending on your need and the stage of product development. Biological molecules are routinely measured using UV absorbance to obtain critical information on concentration and sample purity. DNA measurements typically involve samples at the extremes of the concentration range with DNA suspended in solution at either very high or very low concentrations. It is important to note that with STS-UV, this entire concentration range was measured using a 1 cm pathlength cuvette.
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With a wavelength range of 190 to 650 nm and approximately 1.5 nm optical resolution, the STS-UV microspectrometer is ideal for these measurements.
While dilution can be done with concentrated DNA solutions, low concentration samples present more of a challenge.


DNA absorbance was measured in a 1 cm pathlength cuvette with an STS-UV and the deuterium lamp of a DH-2000-BAL balanced, deuterium and tungsten halogen light source. Replicate measurements at these concentrations showed variability of less than 0.0004 AU (error bars fall within the data marker). The linearity of the data over this wide concentration and absorbance range is illustrated by an R2 value of 0.9996. Ideal for DNA, RNA and protein determination, STS-UV covers the entire range from 190 to 650 nm with approximately 1.5 nm optical resolution.
The STS-UV packs tremendous performance into a compact, powerful microspectrometer to provide high quality UV absorbance data over a wide range of sample concentrations.
Without the benefit of an adjustable pathlength spectrometer or small volume sampler, the cuvette pathlength itself must be adjusted to enable measurement of a wide range of concentrations. Measurements were made with both the deuterium and tungsten halogen lamps and with just the deuterium lamp to show the impact of out of band light on the maximum absorbance level achieved with the spectrometer.


The linearity of the low concentration data is demonstrated by a correlation coefficient value of 0.9999 for a linear least squares fit of these data points. As shown in Figure 1, STS-UV provides repeatable, high quality DNA absorbance data even with absorbance values as low as 0.005 AU. STS-UV simplifies UV absorbance measurements by enabling measurements over a wide concentration and absorbance range without the need for adjustable sampling accessories.



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