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Add important lessons to your Custom Course, track your progress, and achieve your study goals faster. The transposon-display technique for detecting new transposable element (TE) insertions is illustrated through the use of an example of stress-induced mobilization of the Tnt1 retrotransposon in tobacco.
Induction might be mediated by the binding of a transcriptional activator to a cis-motif (yellow box) in the LTR62.


Some Tnt1-encoded mRNAs are converted into double-stranded cDNAs that integrate into the tobacco genome.
Adaptors (pink boxes) are ligated to all fragments and the mixture is used as template for one (as shown) or two (not shown) round(s) of PCR amplification, using primers that are complementary to subterminal TE sequences (arrows). In this example, DNA was isolated from tobacco plants either before treatment (lanes 1,6) or after regeneration from protoplasts (lanes 2–5,7).






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