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Many years ago when I was offered a job at the University of Texas Medical Branch, I proposed several studies. Add that to previous studies showing probiotics reduce allergies and lower the rate of one of the most serious problems newborns suffer (necrotizing enter colitis). A range of studies and scientific evidence about why so many people are getting fat have been building towards this. The principle attention regarding specific probiotic species and weight loss is focused on the relative balance of two species (firmicutes and bacteroidetes).
Observing a pattern does not establish causation, nor does it prove that changing the bacteria in our intestines will cause weight loss or gain.
Eventually I believe that we will identify the most effective balance of healthy bacteria for each individual. Michael Carlston, MD is an internationally recognized authority in the integration of conventional and complementary medicine in clinical practice, as well as medical education, research and organizational consulting. We will be provided with an authorization token (please note: passwords are not shared with us) and will sync your accounts for you.
The gut immune system is influenced by many factors, including dietary components and commensal bacteria. The gut associated lymphoid tissue is the largest in the body, and mature lymphocytes in the gut mucosa vastly outnumber those in the bone marrow (1).
The contributions of the gut microbiota to the development of the immune system have been extensively characterized. Strategies that try to restore the normal gut microbiota have been extensively studied in human and animal models, as these methods represent a valuable tool to treat several disorders. The rapid growth of metagenomics strategies is being of great help to understand the role of specific microorganism and the overall diversity of the microbiota in many human diseases. The idea that bacteria in the gut could play a role as a regulator of health and disease was first proposed by Elie Metchnikoff more than a century ago.
Fukuda and collaborators (149) showed that the probiotic Bifidobacterium protects gnotobiotic mice from death induced by enterohemorrhagic E.
Our research group also investigated certain probiotic properties of different microorganisms (Bifidobacterium animalis var.
Although most of the effects of probiotics are beneficial, several negative effects should be considered before therapeutic application. Fiber carbohydrates (including cellulose, pectin, gums, beta-glucan, and lignin) are not digested in the upper gastrointestinal tract because the host does not have the enzymatic capability to degrade these carbohydrates (157).
Diet alone has the strongest and most direct effects on gut microbial colonization because bacteria have different preferences for different energy sources. Stomach of a newborn baby is very delicate, the only food for him is breastmilk or infant formula. As the baby grows, his digestive system will develop slowly and able to eat select foods (starting from soft biscuits, potato, squash and carrots). According to its manufacturer, Yakult contains billions of probiotic bacteria, the Lactobacillus casei Shirota. NEVER trust a product with no Contact Details (Customer Service) on their package, particularly Yakult. Altaflora is suitable for children as from newborn and can be mixed with milk in cases when diarrhea is not present. Altaflora Electrolytes sachets is also used in many European countries apart from a treatment of the above to also flatten the belly and keep a good figure. Patients with inappropriate diets, stress, aging, antibiotic therapy, peristaltic disorders that cause indigestion, flatulence, constipation, diarrhea, gastro-enteritis. In order to receive information about your Personal Data, the purposes and the parties the Data is shared with, Alta Care Laboratoires and its partners are using cookies. One of those was to administer probiotics to newborn babies to see if we could reduce the rate of colic.
My decades-long advice to parents, advising them to start probiotics in pregnancy and give them to their newborn babies, seems better and better.
Undoubtedly the problem is far more complicated and many of the factors, like environmental contamination with endocrine disrupting chemicals, we can’t change easily or quickly.
A new study, using a bacteria closely related to the Culturelle probiotic I have been using in my office for twenty years, found just that but I don’t think it is quite so simple. Representing the sum of my experience as well as the latest clinical research, my perspective is often somewhat at odds with the simplistic articles appearing in the media.
This means that you will not need to remember your user name and password in the future and you will be able to login with the account you choose to sync, with the click of a button. This page doesn't support Internet Explorer 6, 7 and 8.Please upgrade your browser or activate Google Chrome Frame to improve your experience. Nutrients that affect gut immunity and strategies that restore a healthy gut microbial community by affecting the microbial composition are being developed as new therapeutic approaches to treat several inflammatory diseases. Large amounts of antigens pass through the gut daily, making the gut mucosa the major site of lymphocyte contact with antigens in the entire body. These partners act similarly to an organ that can provide nutrients and help the host to digest foods, including extraction of additional calories from and metabolism of complex carbohydrates to generate short-chain fatty acids (SCFAs). There is coordinated cross talk between the gut microbiota and the immune system, allowing the host to tolerate the large amount of antigens present in the gut. A randomized clinical study was the first to report that restoring a healthy gut microbiota is a logical strategy for treating enteric infection (15).
This knowledge can help the development of therapies focused on specific effects of different probiotics and prebiotics on the gut microbiota.
Metchnikoff proposed that toxins produced by a putrefactive microorganism in the colon could inhibit the growth of other bacteria. Probiotics have been long reported to aid in the treatment of many dysfunctions of the GI tract, and the mechanisms by which probiotics work have recently been elucidated (Table 3). Main mechanisms of action of the major pharmaceutical probiotic products for human purposes. The most important concern about probiotic use is the risk of bacteremia, fungemia, and sepsis (155). Currently, only inulin and galacto-oligosaccharides, which are natural food ingredients that are present in certain plants as storage carbohydrates, fulfill all of the criteria for prebiotic classification. However, these substances are thought to be selectively ferment by residential bacteria into SCFAs, and particularly acetate, propionate, butyrate, and lactate, once in the colon (158). Altaflora Electrolytes sachets is a treatment for inappropriate diets, stress, aging, antibiotic therapy, peristaltic disorders that cause indigestion, flatulence, constipation, diarrhea, gastro-enteritis.

In many European countries doctors prescribed one sachet of electrolyte sachets and 1 sachet of probiotic sachets containing flora every 8 hours in cases of diarrhoea and dehydration. Animals, including humans, that live in colder climates tend to be larger, with higher levels of body fat. The “best” for one person will probably change along with other chronic and evolving health factors. Carlston was voted “Best General Physician In Sonoma County, California” by readers of the Sonoma County Independent newspaper and also named one of the outstanding physicians in the Bay Area by San Francisco Focus Magazine. Although probiotics (live microorganisms) and prebiotics (food components) have shown promise as treatments for several diseases in both clinical and animal studies, an understanding of the molecular mechanisms behind the direct and indirect effects on the gut immune response will facilitate better and possibly more efficient therapy for diseases. In addition, approximately 100 trillion bacteria are associated with our gastrointestinal tract. Furthermore, the gut microbial community is akin to a safeguard of our health because the microbiota compete (for space and nutrients) with potential pathogens and induce the secretion of antimicrobial peptides through interaction with intestinal epithelial cells (5, 6). The researchers showed the effectiveness of transplanting feces from a healthy patient into patients with recurrent Clostridium difficile infections, which can cause severe diarrhea (15). He came across research noting that a certain rural Eastern European population whose staple was large consumption of fermented milk had unusually long lives. Clinical trials have shown that the use of probiotics in the prevention of diarrhea can be efficacious especially in newborn and children (44–46). The Escherichia coli strain Nissle 1917 has been demonstrated to improve intestinal homeostasis and to minimize the bacterium-induced reduction of the intestinal barrier, thus decreasing the invasion of intestinal epithelial cells by several pathogens.
Thus, use of probiotics in immuno-compromised patients could generate serious health risks. Although the previous definition of prebiotics is only applicable to selectively fermented food components, and although much of the prebiotic literature focuses on non-digestible oligosaccharides, most dietary fibers that are fermentable carbohydrates could be considered as prebiotics as well.
The vast majority of the bacteria in the colon are strict anaerobes that derive energy from fermentation. The profile of dominant species in the human gut microbiota can potentially be modified by dietary intake, with consequences for health. However now with Altaflora electrolyte sachets by Alta Care Laboratoires it is possible to have both electrolytes and flora in one sachet.
The probiotic (Lactobacillus reuteri DSM 17938) cut episodes of inconsolable crying by about half.
As the cold environment demands burning calories to keep warm, and fat is a rich source of calories, this would seem to be a good adaptation. This geographic study also hints that the ideal for a person might also change depending upon where they live at the time. In this review, we will first describe the concept of prebiotics, probiotics, and symbiotics and cover the most recently well-established scientific findings regarding the direct and indirect mechanisms by which these dietary approaches can influence gut immunity. This rich gut microbial community referred to as the microbiota has coevolved in a symbiotic relationship with the human intestinal mucosa in such a way that the indigenous microbiota is essential for gut homeostasis (2, 3). The gut microbiota can also stimulate the differentiation and proliferation of epithelial cells, which regulate intestinal homeostasis (7–9).
The advances in current knowledge of gut microbial biodiversity allow us to understand the mechanisms of how different microorganisms influence host function and these mechanisms’ impact. The composition of the gut microbiota varies during childhood, until the individual reaches adulthood. Acute diarrhea is the main symptom of acute gastroenteritis whose most common etiologic agents in children under 1 year are Rotaviruses.
In this review, we highlight the most commonly used probiotic products and the recently described mechanisms in humans (Tables 1–3).
Probiotic research is moving forward due to basic science and clinical trials evaluating the safety and efficacy of probiotics for various medical conditions. We hypothesize that any type of dietary or food supplement that could promote the growth of beneficial bacteria and consequently promote homeostasis in the gut and good health could be considered as a prebiotic, even though the supplement may not meet the required criteria.
The gut microbiota can ferment fiber due to their expression of several enzymes and transport proteins.
The two most abundant phyla found in most healthy individuals are the Bacteroidetes and Firmicutes (160, 161). Nevertheless, the delivery of SCFAs has been shown to be successful and effective in reducing colitis (183, 184) and other colonic inflammatory disorders (185). Also, with the plethora of food sources available in tropical environments, animals do not need to store calories. Matching the inner ecosystem of our bodies to the outer ecosystem of the world immediately surrounding us, is a very cool concept. Emphasis will be placed on the relationship of diet, the microbiota, and the gut immune system.
Altered microbiota (dysbiosis) are associated with gastrointestinal disorders, but more recently, we observed microbial imbalance associated with broad diseases that are not restricted to the gastrointestinal tract (10, 11). He isolated Bacillus bulgaricus and promoted its use as a therapy to maintain homeostasis and prevent aging, thereby popularizing yogurt (37), which formed the foundation for probiotics. Several studies have been show that pattern recognition receptors (PRRs), Toll-like receptors, and Nod-like receptors, have crucial roles in maintaining a healthful stable relationship between the host gut and its microbiota (28, 40). There is strong evidence of the clinical benefit of Lactobacillus and Saccharomyces boulardii treatment to decrease the duration of diarrhea (see Table 2) (45).
Nevertheless, additional research is still needed to elucidate the functional aspects and the detailed mechanisms of action of probiotics and their impact on human health in relation to various diseases. However, clinical trials of probiotics still have limitations and further studies about the amount and interval time are still required cause the benefits of probiotic action vary according to that. Dietary fibers can act as effective prebiotics by inducing major shifts in gut microbial composition and directly affecting the mucosal immune system, resulting in an improvement in enteric inflammatory disorders and the systemic immune response (see Table 4). The symptoms usually last from several days to two weeks.  I was not sure if the cause was the coliform bacteria or there are other unknown substance in water. A worldwide study of 1,200 people found that the balance of firmicutes and bacteroidetes paralleled those long-established observations.
Second, we will highlight recent results from our group, which suggest a new dietary manipulation that includes the use of nutrient products (organic selenium and Lithothamnium muelleri) and probiotics (Saccharomyces boulardii UFMG 905 and Bifidobacterium sp.) that can stimulate and manipulate the gut immune response, inducing intestinal homeostasis.
The newest evidence shows that the gut microbial composition is associated with malnourishment (which causes one of the highest rates of child mortality in the world) in children from Malawi in southern Africa (12).
The dynamic state of the microbial ecology is increasingly being associated with an expanding number of disorders. TLR activation results in the up-regulation of pro-inflammatory mediators that facilitate host’s immune defense responses.

Diarrhea associated with antibiotics administration is also frequent affecting up to 30% of newborn children (47).
Different probiotic strains exert their beneficial effects via various different mechanisms and may be synergistic with other microbiota. Butyrate is the major energy source of colonic epithelial cells affects the proliferation and barrier function of the colonic epithelium and reduces oxidative DNA damage (186, 187). The closer people lived to the equator, the greater the predominance of bacteroidetes in their digestive tract.
Furthermore, the purpose of this review is to discuss and translate all of this knowledge into therapeutic strategies and into treatment for extra-intestinal compartment pathologies. In this work, Smith and colleagues transplanted the gut microbiota of a malnourished twin with kwashiorkor into germ-free (GF) mice (which are devoid of microbiota) and observed that these animals lost more weight than mice transplanted with the healthy twin’s bacteria. However, the importance of perturbations in the gut microbiota and the subsequent impact on the development of inflammation has only recently been recognized. The NLRs are cytoplasmic proteins that regulate inflammatory responses and activation of these PRRs by commensal microbiota has been evolved to contribute to gut homeostasis (41). Diet supplementation with Bifidobacterium lactis and Streptococcus thermophilus was shown to reduce the frequency of antibiotic-associated diarrhea (AAD) in infants (48). One probiotic strain may have a different set of properties and clinical effects than another probiotic strain, even if the strains are of the same genus and species.
This energy source is transported into cells via monocarboxylate transporters, such as MCT-1107 (188). We will conclude by discussing perspectives and molecular advances regarding the use of prebiotics or probiotics as new therapeutic strategies that manipulate the microbial composition and the gut immune responses of the host.
Previously, the association of the gut microbiota with metabolic diseases was demonstrated by the same group, which showed that alterations in the gut microbiota could affect human obesity (13, 14). Therefore, ecological principles such as colonization, succession, resistance to change, competition, and cooperation between community members of the microbiota are beginning to be explored (6). However, perturbations of PRR-microbiota interactions, in different cells type and gut mucosal compartment, are more likely to promote disease states associated with exacerbated inflammation (42, 43).
Increased production of short-chain fatty acids in the colon that stimulates the absorption of sodium by the colonocytes as well as a decrease in intestinal permeability and invasion of pathogenic microorganisms have been proposed to be the major mechanisms by which probiotics reduce diarrheal symptoms (47, 49). Thus, it is important to note that the efficacy of one probiotic strain does not imply that the other strains will be equally efficacious; rather, further research needs to be performed.
The researchers found that this effect can be attributed, at least in part, to increased production of acetate by the protective bifidobacterial strains, which improves the intestinal defense mediated by epithelial cells (150).
Butyrate has been shown to reduce the incidence of colon cancer by inhibiting histone deacetylases (HDACs), which affects binding to DNA and thereby transcriptional activity (189).
However, the composition and functional characteristics of a healthy gut microbiota remain to be elucidated. In the post-genomic era, new high-throughput methodologies such as metagenomics, transcriptomics, proteomics, and metabolomics have greatly help the understanding the mechanisms by which the microbiota contributes to host physiology in healthy and diseases. Butyrate, which is more highly produced on a resistant starch, soluble fiber, and inulin diet, was also associated with increased percentages of T-regulatory cells (Treg) and reduced production of IFN-γ, suggesting a down-regulation of inflammation, in an experimental model of IBD (190). Metagenomic studies of the human gut microbiota, for example, have suggested that low bacterial diversity affects host metabolism and is related with obesity and other diseases (4, 16, 17). The microbiota is essential to prevent the attachment, growth, and penetration of pathogenic microorganisms on the gut surface. The intestinal microbiota play an important role in pathogen resistance, both by direct interaction with pathogenic bacteria and by influencing the immune system (6, 18). The commensal bacteria that reside in the gut are diverse, and in certain cases, individual species appear to have distinct and opposing roles in the gut immune response. Certain commensal bacteria appear to preferentially drive T-regulatory lymphocyte development, whereas other bacteria promote Th17 development in the gut (19). An investigation of the mechanism by which different probiotic strains trigger a reaction could help to indicate the best microorganism for therapeutic and prophylactic application in several inflammatory diseases.
Much evidence for how the microbiota shape the immune system comes from studies in GF mice, which completely lack microbiota.
In addition, it is important to highlight that probiotic properties are not only attributed to bacteria. Previous results from our group showed that treatment with yeast Saccharomyces cerevisiae strain 905 significantly reduced the translocation and dissemination of pathogenic Salmonella typhimurium to all organs in GF and conventional mice after oral infectious challenge (151). The protection conferred by the probiotic against pathogenic bacteria that was observed in this study was most likely due to modulation of both the intestinal and the systemic immunity of mice treated with the probiotic yeast.
Consequently, serum immunoglobulin (Ig) G and intestinal IgA levels in GF mice are reduced (23–25).
Additionally, many studies in mice and humans indicate that certain inflammatory diseases are associated with an altered microbiota (26–29).
A prospective study of children with a high risk of developing asthma suggested that changes in the microbiota occur before disease development (30). Furthermore, Matins and collaborators demonstrated in vitro and in vivo that probiotic therapies could be useful as adjuvant when treating gastrointestinal diseases. It is becoming clear that certain species of gut commensal microbiota are required for the regulation of immune responses and that perturbations in the microbiota could result in a lack of immune regulation, the outgrowth of more pathogenic microbes, and the promotion of inflammation. The microorganism to which the newborn child is exposed during the first years of life will mainly determine the microbial composition of the microbiota in the adult human gut.
Indeed strategies to manipulate the microbiota during infancy have been shown to prevent development of allergic and atopic diseases later in adult life (31–33). Thus, the use of probiotics and prebiotics during the early postnatal period has been proposed to intentionally modulate the microbiota composition. In addition, diet and exposure to microbes during pregnancy may influence the metabolic and immunologic profiles of the pregnant uterus and the risk of disease development in the offspring (34).
Although most of the conditions under which the useful therapeutic application of probiotics were first described were in the gastrointestinal tract, demonstrating regulation of gut immunity, how probiotics could alter host physiology and function in systemic disorders started to be explored once it became apparent that microbial-immunologic relationships with the host may have implications in extra-intestinal systems.
Thus the administration of probiotics and prebiotics during pregnancy has also been proposed. The influence of probiotics and prebiotics on the gut microbiota in both maternal and infant health has been the subject of recent studies (35, 36).

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Category: Probiotic For Children

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