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Step 2: Prepare Clean RoomTo perform the grain inoculations, you require a sterile environment. Step 6: Inoculation I (Grain Spawn Transfer)There are a number of methods to inoculate the sterilised grain. Step 7: Inoculation II (Agar Tissue Culture Transfer)Another method to inoculate your grain, is by first propagating the mushroom tissue on Agar (or cloning).
Step 8: Inoculation III (Liquid Inoculation Methods)One simple way of making your purchased mushroom spawn go further, is to add it to sterilised water, making a liquid inoculant. Step 9: Inspect JarsWith grain spawn transfer, you should notice popcorn sized colonisation evenly spread throughout the grain after 1-2 days. After the spider finds its prey, it expels digestive enzymes from its intestinal tract onto the prey.

Air is full of impurities and so it is important to reduce the level of containments where possible. Sharks have U-shaped stomachs that use very strong acids and enzymes to dissolve most of what is eaten. This instructable describes how to propagate oyster mushroom spawn via grain spawn transfer, agar tissue culture transfer and liquid inoculation methods. These methods are all low tech (requiring only basic equipment), covering the pleurotus ostreatus (winter) and pleurotus pulmonarius (summer) varieties.
Only this liquid mush enters the intestines because the pyloric valve (the valve between the stomach and the intestines) is small.
A clean cabinet is a very good idea to avoid contamination and you could add an air filter (hepa - pass-through) to help avoid contamination if you find that to be a problem.

I'm not sure why but working with spores seemed a less vigorous and reliable method for me (or perhaps I was too impatient), it worked but often resulted with a high contamination level. This method is best to promote diversity (and actually should result with strong vigorous mycelium networks) but relies on good spawn capture methods. Can I just get a mushroom from the market and take a piece from that to inoculate the agar?
That is a test tube full of agar with a bit of wood in it that can keep viable in the fridge for years.

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