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Author: admin, 06.06.2016. Category: Garden Soil

Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Clipping is a handy way to collect and organize the most important slides from a presentation. Pyrophosphate ions (PPi), a by-product of the targeted DNA amplification reaction, and APS, are enzymatically converted into ATP by ATP-Sulfurylase. Chemistry Overview Isothermal DNA Amplification • Multiple primers recognize distinct regions of the genome • A DNA polymerase with strand displacement activity • Efficient, rapid and continuous amplification of target DNA Bioluminescence Detection • Exponential generation of pyrophosphate, a by-product of the DNA amplification • Pyrophosphate conversion to Adenosine Tri Phosphate (ATP) • Thermostable luciferase uses ATP to generate light Bst DNA Polymerase PPi ATP Light Light PPi ATP- ATP Luciferase Sulfurylase PPi APS Light Light PPi ATP ATPHow does it work?The 3M™ Molecular Detection System uses multiple primers to recognize distinct regions of the genome and Bst DNA polymerase to providecontinuous and rapid amplification of genetic material.


ATP reacts with luciferase to produce light which is detected indicating the presence of target organism DNA. Pyrophosphate ions (PPi), a by-product of the targeted DNA amplification reaction, and APS,are enzymatically converted into ATP by ATP-Sulfurylase.
Both amplification and detection occur simultaneously and continuously during the exponential phase providing real time results and a short run time. 3M Food Safety products are at work in more than 60 countries around the globe. ATP reacts with luciferase to produce light which is detected indicating the presence oftarget organism DNA.


Our passion is work with food and beverage processors to discover new food safety solutions that protect their brand, mitigate their risk and improve their operational efficiencies. Both amplification and detection occur simultaneously and continuously during the exponential phase providing real timeresults and a short run time.



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