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MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. Using conventional analytical methods, retention of metala€“EDTA complexes is accomplished using ion pair reversed phase chromatography. Your use of this website constitutes acknowledgement and acceptance of our Terms & Conditions.
Chelated micronutrients are widely used in agriculture and are strongly promoted by the hydroponics industry.  But exactly what are they and why should they be used?
In plain terminology, the EDTA complex surrounds the cationic metal molecule and forms weak bonds with it, effectively giving the nutrient a neutral synthetic coating.  Chelates are especially effective for use with micronutrients being applied to alkaline soils. The more bonds that form between the ion and the carbon atoms – either synthetic or organic forms of carbon – the stronger the ion is held within the chelate.
The value of chelation becomes clear when one realizes that many micronutrients are unavailable to plants in their basic forms. As a result, the element cannot enter the plant due to the difference in repelling electrical charges. There are several chelating agents that are commonly used in commercial fertilizers which are able to effectively neutralize the opposing electrical charges present between micronutrients and the root zone.

EDTA is the most widely used chelating agent and is effective for both soil and foliar applied nutrients; it has four molecular points of connection.
The graph below shows the level of iron stability with different chelating agents at different pH levels.
Because it is the strongest and therefore most effective of the synthetic chelating agents, EDDHA is found only in select fertilizer formulations because of its relatively high cost. In addition to humates, there are number of other natural substances used as chelating agents. In addition, ascorbic, boric, and citric acids are all organic acid compounds which provide a degree of mineral chelation by neutralizing positively charged mineral compounds.  These organic carbon acids also play a useful role in vascular mineral transport. Ideally, in order to optimize growth in your garden, the use of several sources of chelation in the nutrient solution is recommended. However, the ion pair agents used in the mobile phase are not compatible with mass spectrometry.
Lignosulfonates, phenols and polyflavinoid chelates are produced using fermented by-products, mainly from the wood pulp industry. However, plant leaves have a waxy coating that protects them from dehydration, and this surface wax also repels water and inorganic substances making it difficult for inorganic nutrients to penetrate into the leaf.

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In this LC-MS method using the Diamond Hydridea„? column, only formic acid is needed in the mobile phase in order to obtain retention of a Zinc-EDTA complex. When the chelated element is required, the plant will remove the positively charged element – such as iron – from the chelate and absorb the element. However, the chelate’s non-ionic coating prevents these reactions from occurring in the soil or other growth media.  Plant roots are then able to absorb and take up the chelated nutrient allowing the chelate to release the nutrient within the plant. When heated to decomposition,Ethylenediaminetetraacetic acid, disodium zinc salt (CAS NO.12519-36-7) emits toxic vapors of NOx and Zn.

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