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The disassembly of MSP fibers can be promoted in the same cell-free system (Miao et al., 2003). MFP2 is a 38 kDa protein that appears to be essential for fiber formation (Buttery et al., 2003).
All WormBook content, except where otherwise noted, is licensed under a Creative Commons Attribution License. Optimizes your chances of conceiving with FertilAid for Women - a fertility enhancing supplement designed for trying-to-conceive women. Information on this site is not meant to substitute for the advice of a physician or medical professional and should not be not used for diagnosing or treating a health problem or disease, or prescribing any medication. In evaluating motility with most species, sperm are classified as non-motile, progressively motile or non-progressively motile. Another term that is sometimes used is "total motility" which refers to the fraction of sperm that display any type of movement. There is considerable variability among species in what is considered normal or acceptable motility. When analyzing motility, it is important to know whether the semen sample has been abused in any way.


Manual motility estimates (wet mount): This commonly-used technique involves placing a sample of diluted semen on a microscope slide, examining it with a microscope and estimating the fraction of the population that is motile. More specifically, a sample of semen is diluted in warm extender or buffered saline, and about 10 to 20 microliters of this sample is pipetted onto a clean, prewarmed microscope slide. A bright field microscope can be used for evaluating motility if the field diaphragm is closed to enhance contrast and ability to visualize sperm.
Track motility estimates require more time to perform that manual estimates, but they are objective and repeatable. Computer-aided motility analysis : A number of systems have been developed that allow motility to be analyzed with the aid of a computer. However, nematode spermatozoa contain no actin (Nelson et al., 1982), but instead employ MSP filaments in this role. Information and statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease. A progressively motile sperm swims forward in an essentially straight line, whereas a non-progressively motile sperm swims, but with an abnormal path, such as in tight circles. Exposure to heat, cold, any kind of residue on collection equipment, or the wrong pH or osmolality of an extender can adversely affect motility.


Identification of a large multigene family encoding the major sperm protein of Caenorhabditis elegans. Dissection of the Ascaris sperm motility machinery identifies key proteins involved in major sperm protein-based amoeboid locomotion. Supramolecular assemblies of the Ascaris suum major sperm protein (MSP) associated with amoeboid cell motility.
Cell-specific transcriptional regulation of the major sperm protein in Caenorhabditis elegans. Genetic and molecular analysis of spe-27, a gene required for spermiogenesis in Caenorhabditis elegans hermaphrodites. Identification of protein-protein interactions of the major sperm protein (MSP) of Caenorhabditis elegans.
Sperm morphogenesis in wild-type and fertilization-defective mutants of Caenorhabditis elegans.



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