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Yet, it does matter!  Studies have shown that in order to optimally benefit and ensure that the good bacteria makes it down into your intestines, timing of probiotics is key! Many people know the benefits of adding probiotics, “friendly bacteria”, to their health regimen and take them either as a supplement or consume them in fermented foods that have active live cultures, such as yogurt, fermented pickles or fermented sauerkraut. However,  how to take probiotics is often not considered, specifically what time of the day is it best to take them. If you are taking a supplement, it is best to take it on an empty stomach when you wake up in the morning or before bed.
A recent study in the Journal of Beneficial Microbes showed that probiotics are optimally absorbed when taken prior to a meal and were absorbed almost as well when taken with a meal containing fats. You don’t have to take probiotic supplements to ensure healthy intestinal flora, you can also make fermented foods! I’ve had the best results with Plexus probiotics, which include enzymes that help break down the yeast overgrowth in your gut.
I have been taking the Lady Soma Probiotics for about 1 year now and I can’t belive the difference it makes in my digestive health! The study in the Journal of Beneficial Microbes says that taken before a meal is almost as good as taken with a meal with fat. It could depend on what type of detox you’re doing, but I prefer to never go without either a good probiotic or fermented foods. I’ve been taking probiotics for years first thing in the morning right before I drink my shake. Additionally, your body requires a daily dose of probiotics to sustain a healthy balance in your gut and digestive system. It's become increasingly clear in recent years what the next major area of exploration in human health and performance will be, and the answer might surprise you: the gut!
Based on what we know right now, the game plan for improving your gut health should include increased consumption of both probiotics and what are known as "prebiotics." Here's how to get them, and why.
Probiotics are tiny, living organisms, like some bacteria and yeast that reside inside our gut. There are plenty of them inside you right now—think 100 trillion, not just millions or billions.
Probiotic organisms are found in a wide variety of fermented foods such as yogurt, kefir, buttermilk, selected aged cheeses, sauerkraut, kimchi, and kombucha.1 Supplementation is also a popular route to increase probiotic intake.
The underlying mechanisms by which gut microbiota can impact metabolism and body composition are still unclear, but it seems clear that there is a relationship. Regardless of why you want to improve your gut health, the most important reason seems to be to optimize the ratio of good bacteria to bad bacteria in the gut.
There is also promising and ongoing research tying gut health to everything from cognitive function to emotional health, but most of this remains in its early phase.
The image of the bacterial buffet in your innards may seem a little remote, but the benefits associated with increased prebiotic fiber intake are real.
Some prebiotics occur naturally in foods such as chicory, Jerusalem artichokes, and a wide range of fruits and vegetables, particularly roots and greens.

Typically, a probiotic should contain several billion microorganisms to increase the likelihood of benefit.13 While several multivitamins now contain probiotics, the dosages are often minimal, so it may be worth your while to take a dedicated probiotic supplement for a while if you haven't before. When it comes to prebiotics, dosages range from 2-20 grams per day.1 The amount you decide to consume will depend on the ingredient, your tolerance, and the desired effect. Take your probiotic according to the label's recommendations and whatever is most convenient for you. Clearly, the research hasn't provided a definitive answer here, so for now, just take your probiotic according to the label's recommendations and whatever is most convenient for you. The advantage of supplements is that they can provide substantial numbers of bacteria without requiring you to drastically change your dietary preferences. Jeremy Partl, MS, CISSN, has worked with all aspects of the population in providing nutrition counseling and education. However, the term can also be used when talking about fermented foods, as they also contain good bacteria. When you first get up, take the probiotic, then get ready for the day preferably waiting at least 30 minutes before you eat breakfast. The reason this may decrease some absorption of a supplement probiotic is that the probiotic ends up spending more time in the stomach.
Probiotics that are taken after a meal have even more difficulty surviving stomach acid levels as this is when your stomach acid is at it’s highest.
It is best to make them yourself and not rely on store bought fermented foods as they are often bastardized and  preserved in salt rather than in lactobacterial-salt.
It’s OK to miss a day here and there, but aim to take them at least five days a week.
The manner in which so many systems and organs work together to maintain health is inspiring and researchers are still struggling to understand all of the specifics. Folks of long ago obtain their probiotic requirements from fermented vegetables, freshly picked wild berries, and kefir. They’re particularly suitable for the individuals that feel digestive distress and the ones that suffer from a compromised immune response. I hate spending money on something and using it not to know if it’s really doing anything for me.
To be clear, this is a relatively new area of inquiry, and there are several factors that may influence the bacterial makeup of our gut that are beyond our control. This is when a few potentially harmful bacterial genera or species predominate over more favorable strains. Seeds such as flax and chia, and some nuts (particularly with the skin on), have also been shown to contain prebiotics.1 That psyllium husk you may have taken to help you move things along in the past? You're trying to increase the overall volume of good bacteria in your gut, so most probiotics will recommend you take a dose multiple times a day.
Heat can kill the bacteria and moisture can lead to premature "activation" from the pills, only to see them die shortly after due to a lack of nutrients. Some supplements contain freeze-dried bacteria within capsules and tablets and do not require refrigeration; however, if ever unsure, storing in the refrigerator is your best bet.

Others have far less, and with the current marketing craze around the term "probiotic," it can be hard to tell which is which. So consume fermented food if you can, and definitely aim to get more prebiotic fiber in your diet. Also, another reason to take them on an empty stomach is because before the journey to the intestines, the bacteria will have more difficulty surviving the harsh stomach acid during digestion, affecting their potency.
A study from last year found that when a probiotic supplement was taken after a meal, extremely low levels of bacteria survived.
My gynecologist also suggested a take a probiotic designed for women, especially since I do not like yogurt. I’m aware of most health myths… How much fermented sauerkraut should I eat on a daily basis? Some probiotics are also capable of killing pathogens, thus improving digestion and preventing conditions like candida or urinary tract infections.
You'll find products containing a wide range of strains in amounts ranging from a billion CFU (colony-forming units) to upwards of 50 billion. You'll find arguments online for both approaches, usually focused on helping bacteria survive the acidic environment of the stomach and arriving intact in the intestines. And the ones that contain the most live cultures are often, shall we say, acquired tastes—and expensive ones at that. The most popular prebiotic-rich supplements include inulin, insoluble corn fiber, and potato starch.
A mixture of trans-galactooligosaccharides reduces markers of metabolic syndrome and modulates the fecal microbiota and immune function of overweight adults. Cholesterol-lowering efficacy of a microencapsulated bile salt hydrolase-active Lactobacillus reuteri NCIMB 30242 yoghurt formulation in hypercholesterolaemic adults. Weight loss during oligofructose supplementation is associated with decreased ghrelin and increased peptide YY in overweight and obese adults. Effects of probiotic supplementation on markers of skeletal muscle damage, perceived recovery and athletic performance after an intense single leg training bout.
An inulin-type fructan enhances calcium absorption primarily via an effect on colonic absorption in humans.
Gut microbiota fermentation of prebiotics increases satietogenic and incretin gut peptide production with consequences for appetite sensation and glucose response after a meal. Galacto-oligosaccharides increase calcium absorption and gut bifidobacteria in young girls: a double-blind cross-over trial.

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