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The Electron Microscopy Laboratory offers a comprehensive analytical, product development and failure analysis service for plastics, rubbers and other polymer based materials. EMLab has international expertise and experience with polymers, biopolymers, composites, copolymers, biodegradable polymers, biological polymers, filled polymers, nanocomposites, polymer degradation, thermoplastics, thermosets, recycling of plastics, rubbers, adhesives and sealants. EMLab is able to provide a one-stop shop for investigations involving polymers with specialist facilities available in polymer characterisation and evaluation including chemical composition, molecular weight, structure, morphology and properties.
We are flexible in our approach and we will strive to understand your needs and problems and exceed your expectations.
We are able to respond quickly where required and we are able to provide live access to some of the instrumentation to facilitate a rapid transfer of information.
EMLab has extensive experience working with large and small organisations within the UK, Europe and throughout the World. Industrial processes such as injection molding, extrusion, and solution casting require fine precision and controlled processing conditions to deliver optimized products and end-use applications. At Arkema Analytical Solutions our scientists have the experience and capabilities to help identify the most effective processing solutions, whether the applications are engineering plastics, composites, blends, coatings and thin films, or biodegradable polymers. The stroma comprises 90% of the total thickness of the human cornea and provides the majority of its principle function. Cardiovascular inflammation has been associated with diseases such as accelerated atherosclerosis, occlusive stroke, and pulmonary hypertension, as well as a co-morbidity of ischemia-reperfusion injury following reconstructive surgery and cardiac transplant rejection. Oxygenator failure, bleeding, and thromboembolism are all complications that can result from platelet activation when a patient is being supported by extra-corporeal membrane oxygenation (ECMO) therapy.
Josh Woolley’s research focuses on VADs as well, but one of his main projects involves studying VAD biocompatibility at the clinical level.
Electrospinning is a technique for generating sub-micron sized polymer fibers that has recently garnered extensive attention in the biomaterials community and which has been applied in a variety of tissue engineering efforts. The controlled delivery of bioactive molecules is a common feature desired for biomaterials used in tissue engineering and regenerative medicine.
Soft tissue engineering applications require accurate descriptions of native and engineered tissue microstructure and their contributions to global mechanical behavior. Extracorporeal membrane oxygenation (ECMO) has been used to provide respiratory and cardiovascular support to more than 26,000 neonatal and pediatric patients over the last 25 years, for periods from a few days to a few weeks. The Centre offers expertise in the design of polymeric systems, polymer modification, polymer processing and properties of polymeric systems. We are able to provide the specific microscopy element of a project or undertake a complete programme of work. We are able to work on a confidential fee-for-service basis or in a collaborative programme.

Our polymer microprocessing laboratory is equipped with micro compounders and micro extruders, which permit the study of processability and extrudability of polymers at the laboratory scale for a quick and accurate identification of common processing problems.
Kazuro Fujimoto is directed toward tissue-engineering approaches that seek to maintain or recover cardiac function following ischemic myocardial damage (i.e. Yi Hong’s research interests focus on biomaterial synthesis and processing for drug delivery and tissue engineering. Zuwei Ma’s primary research areas is the synthesis of bio-absorbable thermally responsive hydrogels for soft tissue injection, with applications in cell therapy and drug delivery. Sang-Ho Ye’s research seeks to develop technologies that reduce the thrombogenicity of cardiovascular device surfaces by generating a stable, hemocompatible interface between the metallic materials and the blood. Ryotaro Hashizume’s research is on abdominal wall repair and regeneration using elastic, biodegradable scaffolding materials such as poly(ester urethane) urea (PEUU).
Devin Nelson’s research is concerned with studying how gene-inducing molecules and growth factors can be incorporated into and released from relevant tissue engineering scaffolds.
Antonio D’Amore’s research project focus on the development and experimental validation of a multi-scale modeling strategy to: (A) guide tissue engineering scaffold design, (B) provide a better understanding of cellular mechanical and metabolic response to local micro-structural deformations.
Activation of the hemostatic system by the extensive synthetic surfaces of the pump and oxygenator system used in ECMO leads to major complications for the treated children.
Such technologies are particularly needed for devices where patients are at risk for thromboembolism and must take anticoagulants or anti-platelet therapy. This effort is targeted to developing repair strategies following abdominal compartment syndrome and for fascial reconstruction in general. In this capacity, VADs have proven to be a beneficial treatment modality for bridging patients until the time that they can receive a heart transplant as well as to provide long term circulatory support for patients who will not ultimately be transplanted. The introduction of rotary blood pumps decreased the size and power requirements and increased the mechanical life of VADs, expanding the size of eligible patient population and increasing the quality of life for VAD patients.
His current work involves 1) the release of gene-inducing molecules for spatiotemporal control of gene expression, 2) the controlled delivery of growth factors from porous, elastomeric polymer scaffolds that have been used as a cardiac patch to support cardiac healing after a heart attack, and 3) the release of bioactive agents from thermoresponsive, injectable polymers.
When blood comes into contact with surfaces of the ECMO circuit, platelets may become activated and upregulate adhesion molecules. His efforts have involved the application of both biodegradable, synthetic materials as well as stem cell-seeded scaffolds. Recently, the work has focused on covalent attachment of zwitterionic phosphorylcholine or sulfobetaine moieties, which have previously been shown to exhibit excellent blood biocompatibility. A variety of scaffold processing techniques are being evaluated, including the utilization of muscle-derived stem cells to facilitate repair, and the incorporation of serum factors. The development of pediatric VADs (PVADs) has lagged behind the progress of adult technology, but could benefit thousands of young children suffering from heart failure.

Despite these technological improvements, patients with VADs are still vulnerable to hemostatic complications such as bleeding, thrombus formation and embolization, and infection.
Devin is exploring a variety of methods to achieve increased duration of drug delivery and improved clinical applicability of the scaffolds under study.
A custom-made software was developed and tested on electrospun poly(ester urethane) urea (PEUU) scaffolds to fully characterize engineered construct morphology. For purely biomaterial approaches to cardiac failure, elastic and injectable materials are being evaluated for their ability to mechanically alter the healing environment of the infarcted ventricular wall to result in more optimal tissue remodeling.
Bi-axial mechanical property measurements, histological and genetic approaches are employed to assess the quality of the repair and regeneration.
Recently, several PVADs including the PediaFlow (University of Pittsburgh consortium) and the Levitronix PediVAS have been developed to meet this need and are undergoing testing at the University of Pittsburgh. The detected material topology has been adopted to generate statistically equivalent scaffold mechanical models.
His research has been published in both clinical cardiovascular journals as well as in the biomaterials literature. Jian Wu’s project aims to develop the biological equivalent of human corneal stromal tissue by combining human corneal stromal stem cells (hCSSCs) with a multilayered biodegradable matrix constructed from a polymer designed for this purpose.
One goal of this effort is to create regenerated tissues that closely match the native abdominal wall tissue.
The aim of this project is to develop platelet and leukocyte activation assays that could be used to assess biocompatibility of these new PVADs prior to clinical testing. Our ultimate goal is to connect (1) tissue engineering scaffold fabrication parameters, (2) micro architecture (3) organ level - cell level mechanical response. Gaining a better understanding of how these devices impact cellular elements in the blood will allow for design modifications that we hope will lead to PVADs with excellent biocompatibility and minimal blood trauma entering the clinical arena to aid thousands of children with failing hearts. The model prediction will be used to design and generate prescribed electrospun elastomeric scaffolds. Fujimoto has also been involved in the assessment of novel biomaterials for other applications including abdominal wall and other fascial repair. A mechanistic understanding of how the material micro structure translates into a specific mechanical response would lead to a better performing generation of tissue engineered constructs.

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