Benedict's test to detect the presence (or absence) of sugar in the urine is extremely simple and should be learnt by every diabetic.
Holding the test tube holder, heat it over a spirit lamp until the boiling point of solutions Benedict without overflow. Even if detected in the urine sugar by Benedict's test, the diagnosis of diabetes must be confirmed by blood tests.
Many students confuse the Benedict's Test for Non-Reducing Sugars with the Benedict's Test for Reducing Sugars, perhaps because some of the steps in the procedure are similar. A liquid food sample does not need prior preparation except dilution if viscous or concentrated.For a solid sample prepare a test solution by crushing the food and adding a moderate amount of distilled water.
The test for non-reducing sugars is often conducted on a food sample which tested negative for reducing sugar.If reducing sugars have been shown to be present, a heavier precipitate is often observed when the test for non-reducing sugar is conducted. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. This is a very simple and effective method of ascertaining the presence or the amount of glucose in the urine and can be done by the diabetic himself.
Holding the test-tube with the holder, heat it over a spirit lamp till the Benedict's Solution boils without overflowing.
Even if sugar is detected in the urine by Benedict's test, the diagnosis of diabetes should be confirmed by blood-analysis.


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Even when sugar has been found to be present in the urine, the diagnosis of diabetes should be confirmed by blood-sugar estimation. In the initial stages of the disease, diabetes will not lose their sugar in the urine when your stomach is empty. As non-reducing sugars do not have the aldehyde group, they cannot reduce copper (I) (blue) to the copper(II) (red).Sucrose is the most common disaccharide non-reducing sugar. Sodium hydrogen carbonate is necessary for neutrallization as the reduction of the copper(II) ions will not take place in acidic conditions - of excess acid is present. The blue copper(II) ions from copper(II) sulphate are reduced to red copper(I) ions by the aldehyde groups in the reducing sugars.
Sodium carbonate provides the alkaline conditions which are required for the redox reaction above.


Sodium citrate complexes with the copper (II) ions so that they do not deteriorate to copper(I) ions during storage.
However it is less popular as it less sensitive and requires that the reagents - Fehling's solutions A and B - be kept separate until the experiment is carried out.
In the initial stages of the disease, a diabetic does not lose sugar in his urine, when on empty stomach. This accounts for the colour changes observed.The red copper(I) oxide formed is insoluble in water and is precipitated out of solution. Hence if the Benedict's test is performed in the fasting state, it is possible to miss the diagnosis of the disease. This accounts for the precipitate formed.As the concentration of reducing sugar increases, the nearer the final colour is to brick-red and the greater the precipitate formed.



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