Uridine diphosphate glucose (UDP-glucose) is the immediate precursor for glycogen synthesis.
Glucose-6-phosphate dehydrogenase (G6PD) is a type of protein, called an enzyme, that helps red blood cells work properly. Glycogen synthesis, which occurs through a different metabolic pathway than its breakdown, restores its reserves in the liver and muscle when dietary carbohydrates are available. In the liver it occurs in the fed state and it is stimulated both by increased glucose availability and insulin. In post-exercise muscle, glycogen synthesis is faster when there are high blood glucose levels and insulin is available; the hormone stimulates glucose transport into muscle cells, thanks to the mobilization of specific transporters called GLT4, and glycogen synthesis activity. Glycogen synthesis requires more energy than that recovered during its breakdown: two ATP molecules are spent versus only one ATP molecules saved thanks to glucose-1-phoshate production.
The first step in glycogen synthesis is glucose activation to glucose-6-phosphate, in the reaction catalyzed by glucokinase in the liver and hexokinase in the muscle and the other organs and tissues.


When glycogen synthesis is occurring phosphoglucomutase, the same enzyme that acts also during glycogenolysis catalyzing the conversion of glucose 1-phosphate to glucose-6-phosphate, shifts the phosphate group from C6 to C1 (therefore the enzyme catalyses a reversible reaction). The next step is UDP-glucose production from glucose-1-phosphate and UTP in the reaction catalyzed by UDP-glucose pyrophosphorylase; the reaction, reversible (and the name of the enzyme is due to the reverse reaction), becomes irreversible thanks to the rapid hydrolysis of pyrophosphate to inorganic phosphate in the reaction catalyzed by pyrophosphatase. It should be noted that thus far 2 ATP molecules are consumed per molecule of glucose activated to UDP-glucose: one for glucose-1-phosphate production and the other for the re-synthesis of UPT from UDP in the reaction catalyzed by nucleoside diphosphate kinase.
Then glycogen synthase transfers the activated glucose to 4’-OH group of a glucose residue (a nonreducing termini) present in the molecule catalyzing the formation of an ?-(1,4) glycosidic bond and therefore extending the chain by one glucose unit.
The branches are inserted in the reaction catalyzed by the branching enzyme, also called amylo-?-(1,4)>?-(1,6)-transglucosidase, that catalyzes the transfer en bloc of an oligosaccharide of six to seven glucose units from a nonreducing termini of a newly elongated chain of at least eleven units to another chain forming a new ?-(1,6) glycosidic bond. The new branches are introduced at least at four glucose residues from an adjacent branch point. Therefore, the energy cost that the cell pays to store glucose as glycogen is an high-energy phosphate bond for each glucose unit.


The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood. Next, the health care provider gently inserts a needle into the vein. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding. In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed.
The blood collects into a small glass tube called a pipette, or onto a slide or test strip.



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Comments

  1. 27.05.2015 at 11:12:55


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    Author: HsN
  2. 27.05.2015 at 12:43:30


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    Author: orik
  3. 27.05.2015 at 11:53:53


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    Author: A_Y_N_U_R
  4. 27.05.2015 at 15:37:33


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    Author: Bezpritel