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14.06.2015, admin  
Category: Gh Hormone

Diagnostic Automation offers Testosterone Enzyme Immunoassay (EIA) which is intended for the quantitative determination of testosterone in human serum. The Testosterone EIA is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. The Diagnostic Automation Cortisol ELISA kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Cortisol concentration in human serum and plasma. 17-Hydroxyprogesterone (17-OH progesterone or 17OHP) is a C-21 steroid hormone produced in the adrenal gland and gonads, during thesynthesis of glucocorticoids and sex steroids. Is Testosterone Replacement  Therapy the only answer to increasing low testosterone level ? While the therapies are easy and convenient, there are conflicting reports and concerns that such therapies may backfire and decrease the natural production of testosterone. Testosterone Boosters is an emerging market and we see now new Testosterone Boosters in the market almost everyday. Recommended number of hours of sleep is 7 – 8 hours and it is highly recommended that you sleep at the same time every night. If you are on certain mediation , do review your medication to make sure they are not the cause of low testosterone production. Research published by The Endocrine Society shows that glucose (sugar) decreases testosterone levels in the blood by as much as 25 percent. Changing your daily habits and lifestyle are good for your overall health could also provide benefits in helping to maintain a healthy level of Testosterone. Elle posted on April 23, 2015Thats really good Jovo, I am sure your are enjoying your coffee even more ! There are several options available  to treat Low Testosterone .While some Testosterone Replacement Therapies (TRT) seem effective and easy to administer, each as its own pros and cons . Though most people do not find any difference between the two,  Cypionate is known to have a “slower release” and stays active longer.
The injections are administered into large muscles (usually the buttocks or thigh).  Injections can be given in the clinic or you can be taught by our nurse how to administer them yourself. AndroGel ( also known as TestoGel in some countries ): Testosterone gel (varying strengths).
The advantages of this therapy include convenience and decreased frequency of dosing.  As this requires a short office procedure, there are risks including bleeding, infection, and pellet extrusion in less than 1% of cases. As stated previously, some men shouldn’t take testosterone treatments because they can aggravate symptoms or cause certain health conditions to worsen. There are many things in our everyday life that can lower testosterone and it just takes one weak link to do it .
You don’t need to look like Santa Claus to know you are overweight but those unwanted , extra pounds and kilos are strongly related to low T. Lowering sleep quantity or quality will leads to increased cortisol and a corresponding testosterone decrease . This could well be a result of stress, as it also leads to increased cortisol and inevitably low T. The bar might be a great place to meet your buddies or unwind after work , but more than a few drinks isn’t going to do great things for your testosterone level. There’s a fair amount of research showing that low Vitamin A levels result in lower levels of available testosterone, and that Vitamin A is also crucial in the production of testosterone.
A varicocele is an enlargement of the veins within the loose bag of skin that holds the testicles (scrotum).
Varicoceles are a common cause of low sperm production and decreased sperm quality, which can cause infertility.
When blood sugar levels are high, the pituitary gland produces less LH ( luteinising hormone )     than it normally would. The bad news is that Testosterone level decreases with age and decreases as much as 1% per year after age 30 . Testosterone levels above or below the normal range are considered by many to be out of balance.
While Testosterone is generally associated with sexual characteristics of men , many people are not aware that T is  also responsible for  healthy libido, building muscle mass, and maintaining energy level. Therefore an extreme deviation from the  normal T level should be investigated by a medical doctor.
Men who have Testosterone levels that are higher than average may experience both benefits and drawbacks. I called up 10 male friends above 40 years old and asked them if they ever had a Testosterone ( T) Level Test . Granted maybe they were in super healthy conditions and had no medical problems whatsoever , hence never needed to do a T –Test .
Testosterone testing is used to diagnose several conditions in men, women, girls, and boys.
The best time to take a Testosterone level test is between 7am and 10am when the T level is the highest. Alternatively, there is also a home DIY kit to have your own T level checked by checking your saliva.
The blood spot sample is collected with a finger stick at home, then sent to the lab for analysis. Testosterone is produced by the gonads (by the Leydig cells in testes in men and by the ovaries in women), although small quantities are also produced by the adrenal glands in both sexes.


Present in much greater levels in men than women, testosterone initiates the development of the male internal and external reproductive organs during feotal development and is essential for the production of sperm in adult life.
Testosterone is linked to many of the changes seen in boys during puberty (including an increase in height, body and pubic hair growth, enlargement of the penis, testes and prostate gland and changes in sexual and aggressive behavior). This makes up  40 to 60 percent of our total testosterone is bound to a protein called sex hormone-binding-globulin (SHBG). Measurement of testosterone can be used as an aid in the diagnosis and treatment of various hormonal and sexual disorders. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 10 ul of Testosterone standards, controls, patient samples, 100 ul Testosterone-HRP conjugate reagent and 50 ul rabbit anti-Testosterone reagent at 37C for 90 minutes. 17OH progesterone is a precursor of cortisol that accumulates in the case of adrenal 21-hydroxylase deficiency and decreased after replacement therapy with cortisol. The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen.
Having enough sleep seems to be a major problem with the modern society where 24 hours is not enough.
When you are stressed, your body churns out stress hormone cortisol , the number one enemy of testosterone, and will be less able to produce testosterone. If you are a couch potato, it sends message to your brain that you don’t need so much testosterone to build muscle. I have converted many friends from drinking 3 in 1 coffee to just white coffee without sugar. It’s important to tell your doctor about any side effects if they persist for more than a few days. Pellets, each containing 75 mg of crystalline testosterone, are implanted subcutaneously to provide slow release over 4 to 6 months. Although implantable testosterone, like the injectable forms, can cause levels to peak initially, the decline is gradual, over 6 months, so that mood swings and energy fluctuations are seldom recognized by the patient. Be sure that the spot you have chosen is not oily, hairy, likely to perspire heavily, over a bone such as a shoulder or hip, or likely to be under pressure from sitting or sleeping. Men who have prostate cancer, breast cancer, sleep apnea that is untreated, too many red blood cells (polycythemia), and poorly controlled heart disease should not take testosterone treatments. Oh well , apart from being the cause of of many other ailments , stress is highly associated with low T. Because your body produces most of its testosterone while you sleep, interrupting that cycle with insufficient or poor quality sleep can result in 20% to 30% less testosterone floating around your bloodstream than if you’d gotten enough shut-eye. As your liver processes alcohol, it produces compounds that inhibit the release of testosterone, reducing its overall level in your body and preventing you from doing things like building muscle mass.
Eating the ludicrously high levels of protein that many bodybuilders do will deplete Vitamin A levels and will depress your testosterone levels.
As testosterone is only produced when LH is secreted from the pituitary, this reduces the amount of testosterone produced by a man’s body.
When fighting off a chronic health issue or an infection, for example, your body may simply put less effort into testosterone production. Low T level may result in a decline in physical energy, strength, stamina, and diminished mental aggressiveness. The benefits, which are modest, include normalizing blood pressure and lowering the likelihood of obesity and heart attack. It is also called a serum testosterone test as it measures the total amount of the hormone in the serum , the amount of Free T in the serum and the bioavailable amount – which is the combination of free T and albumin-bound T. Thats what I tell my male friends now and they are surprised cos they did not think …Jovo Vranjes posted on April 23, 2015Interesting test Elle, I myself also have no idea about testosterone level. This hormone also signals the body to make new blood cells, ensures that muscles and bones stay strong during and after puberty and enhances libido both in men and women. SHBG is produced in our livers and plays an important role in regulating the amount of free testosterone in our bodies.
Albumin is a protein produced in the liver, and its job is to stabilize extra-cellular fluid volumes. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody.
Upon mixing biotinylated antibody, enzyme-antigen conjugate and a serum containing the native antigen a competition reaction results between the native antigen and the enzyme-antigen conjugate for a limited number of antibody binding sites. Lack of sleep affects a variety of hormones and chemicals in your body and this, in turn, can have a harmful impact on your testosterone.
However when you are physically active, it send messages to your brain for more of the hormone. Depending on the dose required, 2 to 6 pellets are implanted under the skin of the lower abdomen, upper thighs, deltoid, or gluteal muscles every 3 to 6 months. Furthermore, since their duration of action is long and reversibility is difficult, testosterone pellets are unsuitable for treating elderly patients, in whom adverse effects are more common. Do not apply the patch(es) to the scrotum or to a skin area with open sores, wounds, or irritation. While even one or two drinks will cause a minor, temporary dip in your T levels, this is a bigger worry for individuals who drink heavily multiple times per week.


In essence, your body turns its attention toward the more serious problem, explains Alan Shindel, MD, director of men’s health at the University of California, Davis, Health System in Sacramento.
Studies have shown that total testosterone levels decrease by approximately 30% in healthy men between the ages of 25 and 75.
Moreover, men may experience more aches and pains in the bones and joints and they may also have a decline in libido and erections. However, there is a tendency for such men to consume more alcoholic beverages, and they are more likely to smoke, and more likely to get injured. However if you are on some T therapy , the levels must be monitored closely , at least once in 6 months. In any case if the results are not normal , it is advisable to do a proper blood serum test for T level. After reading your text I might do something …Elle posted on January 3, 2015Thanks Greg !
To effect these changes, testosterone is often converted into another androgen called dihydrotestosterone. Unbound to other molecules, free Testosterone can enter cells and activate receptors in order to work its virile magic on your body and mind.This makes up to 2-3% of your total testosterone level. Albumin-bound testosterone is bound weakly and can easily be broken down in order to create free testosterone. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases.
Serum 17 a-OHP levels are age-dependent, with peak levels observed during fetal life and the immediate postnatal period. A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwells occurs.
Also be sure that the patch will stay flat against the skin and will not be pulled, folded, or stretched during normal activity. I used to wonder why the salesmen of the cross trainers do not recommend over 45 minutes training on the machine ! According to some researchers, the higher the testosterone level, the more likely men are to participate in risk-taking behavior (sexual, injury risk, and even criminal activity). Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter's syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. Levels for both sexes remain at constant low levels during childhood, and then progressively increase during puberty and adulthood. This effects the separation of the antibody bound fraction after decantation or aspiration.
Follow the directions on your prescription label carefully, and ask your doctor or pharmacist to explain any part you do not understand.
Choose a different spot each night and wait at least 7 days before applying another patch to a spot you have already used.
Next, a solution of TMB Reagent is then added and incubated at room temperature for 20 minutes, resulting in the development of blue color.
As with cortisol, serum 17OHP levels normally have an ACTH-dependent diurnal variation, with peak levels in the morning and a nadir at night. The enzyme activity in the antibody-bound fraction is inversely proportional to the native antigen concentration.
The color development is stopped with the addition of 1N HCl, and the absorbance is measured spectrophotometrically at 450 nm. In addition, ovarian production of 17OHP increases during the luteal phase of the menstrual cycle.
By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained. Do not apply more or fewer patches or apply the patches more often than prescribed by your doctor. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled Testosterone in the sample. 17a OHP is a natural progestin and in pregnancy increases in the third trimester primarily due to fetal adrenal production.
A standard curve is obtained by plotting the concentration of the standard versus the absorbance.
Measurements of levels of 17-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective, namely 21-hydroxylase and 11b-hydroxilase, lead to a build-up of 17OHP.
The Testosterone concentration of the specimens and controls run concurrently with the standards can be calculated from the standard curve. In contrast, the rare patient with 17a-hydroxylase deficiency will have very low or undetectable levels of 17OHP.



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