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A blastocyst is an embryo that has developed in culture in the IVF laboratory for at least five days after egg retrieval and has divided into two different cell types. There is now abundant evidence that transferring blastocyst embryos into the uterus five or six days after egg retrieval, results in higher implantation rates per embryo transferred compared to a day three embryo transfer. Traditionally, in an IVF cycle, embryos were transferred to the uterus on the second or third day of development and initial embryonic cell division. Previously, laboratory culture media could only sustain an embryo’s growth for about three days in vitro.
La Jolla IVF is fortunate to have an embryology team that is highly skilled and a laboratory that is technologically advanced. This newer "sequential media" attempts to reproduce the natural environment of the maternal reproductive tract. In general, a blastocyst or embryo transfer procedure is more advantageous for patients who develop a large number of eggs and embryos.
At La Jolla IVF however, many of the patients who are regarded as “poor prognosis” in terms of their ultimate success rate for IVF, choose to undergo what we term “embryo banking” cycles. Over the past several years, patients at La Jolla IVF have completed hundreds of fresh and frozen blastocyst embryos transfers. Medical professionals do not know why some women feel pain when ovulating and others do not. In some women, the ovulation symptoms of pain are a dull achy feeling that lasts anywhere from several hours to two days, but others may only have a single sharp pain in one side of the abdomen that lasts for only a moment.
Some women may be prone to migraines and severe headaches; however, women suffering an increased number of migraines or a surge in severity may categorize their pain as an ovulation symptom. If ovulation pain is accompanied by nausea, vomiting, diarrhea, dizziness, high fever or difficulty breathing, immediate medical treatment should be sought. Women taking fertility drugs may feel severe pain during ovulation due to ovarian hyper-stimulation syndrome.
Ovulation pain is not experienced by all women and is not a reliable indicator of ovulation since it may be caused by indigestion or even appendicitis. The surface cells are termed the trophectoderm and will eventually become the placenta and the inner cells, termed inner cell mass will become the fetus. During the first few days, the embryo relies on the egg cell (from the mother) for all its growing nutrients. An embryo reaching the blastocyst stage has an increased chance of implantation as opposed to a day 3 embryo (all things being equal regarding a healthy uterus). La Jolla IVF’s embryology team has moved systematically towards refining the techniques in the laboratory which now enable many of our patients to avail themselves to transferring blastocysts as opposed to the older more traditional methodology of day 3 transfers. Research throughout the late 90?s identified that as the embryo develops, its nutritional requirements change due to metabolic factors.
It requires much more time and effort to sustain the dynamic process of embryo development to blastocyst stage.


These blastocyst culture conditions do not improve the health or viability of an individual embryo-sequential media does not transform a poor embryo into a good embryo, rather it allows embryos capable of sustained growth to continue growing in the culture media and reach their maximum inherent capability. Because only some embryos are capable of developing into blastocysts, it is possible to have no embryos survive to day 5 to transfer. The optimization of a blastocyst cryopreservation program mandates a comprehensive approach. Not all women experience pain during ovulation and those who do may not recognize the connection between mid-menstrual cycle cramps and ovulation. Ovulation cramps are normally not as severe as menstrual cramps and may occur 7 to 12 days after the first day of your last period.
Doctors and researchers have not been able to identify why this occurs, and unfortunately, it is recommended that women trying to get pregnant forgo their normal migraine medication. These symptoms are associated with serious medical conditions, such as ovarian cysts and appendicitis, which all require immediate treatment. Severe pelvic pain during ovulation or during sexual intercourse may be a symptom of endometriosis or pelvic inflammatory disease. The best way to predict future fertile periods is to use a basal body temperature calendar and to chart all the signs of ovulation each month.
Women who suffer severe pain during ovulation should consult a doctor immediately to rule out more serious medical problems. A healthy blastocyst should hatch from its shell (zona pellucida) by the end of six days or earlier and is then ready to begin to implant within the lining of the uterus.
However, in order to survive post day three or four, the embryo must activate its own genes so that it can carry on growing and dividing. It is believed that the improved implantation rates following a blastocyst transfer is due to selection of the "best" embryos. IVF practitioners were aware that day three embryo transfers were too early when compared to what happens in naturally conceived pregnancies. This research led to the development of different laboratory culture media for the embryo’s specific developmental stages. Our embryologists use specific media to facilitate the embryos development to the blastocyst in vitro during their first three days in culture inside the IVF laboratory.
The ability to develop embryos to the blastocyst stage allows our embryology team to have greater certainty about which embryos are more likely to implant. The embryos that are frozen will be thawed and in combination with the fresh embryos and all the resulting embryos may undergo PGD (not mandatory) on day 3 of in vitro culture (see section on Preimplantation Genetic Diagnosis (PGD) for full explanation).
An appropriate embryo culture system with attention to the protein supplement and a low oxygen environment has been shown to increase the number of blastocysts available for cryopreservation, improve the quality of the inner cell mass and blastocysts post-thaw survival rate.
In our egg donation and surrogacy cases, the ongoing pregnancy rate is over 80% per transfer using blastocysts.
The pain is usually mild to moderate and located on either the left or right side of the abdomen.


This time period is usually indicative of cramps associated with ovulation and can be a good, reliable sign. There are some medical conditions which may cause severe pain, which is not one of the usual ovulation signs and women experiencing excruciating and recurring pain that is severe enough to prevent them from engaging in normal activities should see a doctor as soon as possible. Although most ovarian cysts are harmless and benign, they can cause infertility or infection.
The complications from these medical conditions can be serious so women with severe pain should consult a doctor immediately.
Unfortunately, not all embryos are able to grow past this milestone and in fact only about one third of embryos are capable of becoming blastocysts. In a naturally conceived pregnancy, the embryo reaches the uterus on day four or five because it grows and divides in the fallopian tube for several days after fertilization. These blastocyst culture conditions do not improve the health or viability of an individual embryo, i.e.
The question as to whether the embryos that did not grow into blastocysts could still have implanted if they were transferred on day 3, is not able to be definitively answered at this time.
One of the major limiting factors with doing well with this type of treatment (besides the normal versus abnormal results of the PGD) is the ability of the IVF laboratory to sustain the embryos’ growth in the incubators while PGD is being performed and to enable those embryos that are inherently capable to continue to grow to blastocysts.
These results are being achieved in our program by us routinely transferring a maximum of two blastocyst embryos. There are different types of ovulation pain and cramps, but identifying them can not only be a telling sign of ovulation, but also help women determine the day of ovulation to help or avoid conception.
The pain may be associated with the ruptured ovarian follicle or it may be a side effect of the hormonal changes, particularly the sharp rise in progesterone, that occur during the ovulation cycle. While mild to moderate pain is a sign of ovulation experienced by about 20% of women, severe pain is not normal and a medical professional can determine if the pain is caused by ovulation or a more serious medical problem. Ovulation pain is typically mild and should not be disabling or severe, and is a one of the more common signs of ovulation.
If an embryo can propel itself into becoming a blastocyst in vitro then it would appear that nature has sent us a message that these embryos are the product of a "survival of the fittest" test.
Barry Behr, our Laboratory Director developed one of the first media to successfully grow embryos to the blastocyst stage.
In patients with few embryos on day 3 or where no embryo selection is needed, it is still a rational approach to perform a day 3 transfer (if the patient wishes to do a transfer on their first fresh cycle) as opposed to trying to grow these few embryos to blastocysts and then potentially having no embryos to transfer. This option has dramatically lowered the risk of patients having to deal with the stress, adversity and dilemma of carrying higher order multiples (triplets or more). Our embryology team is constantly upgrading both laboratory equipment and their expertise and we are confident that these advances will ultimately translate into even higher pregnancy rates for patients.



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