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Herpes simplex virus type 2 (hsv-2) igg inhibition elisa, national center for alternative medicine - PDF Review

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Figure 1 Mannosyl-Oligosaccharide Glucosidase (MOGS) Expression and Immunoglobulin Studies in Two Siblings with Congenital Disorder of Glycosylation Type IIb (CDG-IIb). VZV infection is associated with a viremia150 that does not occur during primary HSV-1 infection.
We investigated the role of antibodies against the mature portion of glycoprotein G (mgG-2) of herpes simplex virus 2 (HSV-2) in protective immunity after vaccination.
IntroductionHerpes simplex virus 2 (HSV-2) infects the genital mucosa and establishes a life-long infection in dorsal root sensory ganglia (DRG).
Discussion We show that anti-mgG-2 antibodies, elicited after vaccination of mice with mgG-2 and adjuvant, are important for the outcome after genital challenge with HSV-2.
High levels of immunoglobulin G subclass 1 (IgG1) and IgG2 antibodies were detected and re-stimulated splenic CD4+ T cells proliferated and produced IFN-?. These adjuvants in combination with different subunit HSV-2 antigens given im have been used successfully to elicit robust B- and T-cell immune responses [21,22].
We conclude that anti-mgG-2 antibodies after vaccination significantly increased the production of IFN-? and reduced the viral load in vaginal washes.

Despite high IgG1 titers, none of the sera showed NT activity with or without complement (titer < 20) (Table 1).
Sera from mice that were vaccinated subcutaneously and intranasally with mgG-2 presented significantly lower titers of IgG antibodies and lower ADCC and ACMC activity. An estimate of the global prevalence and incidence of herpes simplex virus type 2 infection. Both gG-2 proteins elicit exclusively type-specific antibody responses in humans [6,7,8,9,10], and mgG-2 is commonly used as antigen for serodiagnosis of HSV-2 infection in clinical settings. We also evaluated whether the immunization protocol modified IgG titers and ADCC and ACMC activity by comparing intramuscular with sc plus in administrations. Cells and VirusesAfrican green monkey kidney (GMK-AH1) cells were cultured in Eagle's minimal essential medium supplemented with 2% calf serum and antibiotics.
The discordant results may be explained by differences in the amount and specificity of antibodies given, virus dose, and the challenge model used. The concentration of mgG-2 specific IgG antibodies was analyzed in an ELISA using a standard curve created by a mixture of the MAbs.

Concluding RemarksData presented in this report suggest that mgG-2 has an important, but to date unknown function, in the genital infection, which can be targeted by immune responses elicited after vaccination. Non-neutralizing antibodies which contribute to protection, possibly mediated by ADCC or other humoral effector functions, have been described not only for HIV, but also for other RNA and DNA viruses [39]. IgG antibodies against mgG-2 were detected by an indirect ELISA as previously described [9,11]. Anti-mouse subclass-specific peroxidase-conjugated IgG were used as conjugates (Southern Biotech, Birmingham, AL, USA) at a 1:1000 dilution.

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